TY - JOUR
T1 - TiO2−MWCNT nanohybrid
T2 - Cytotoxicity, protein corona formation and cellular internalisation in RTG-2 fish cell line
AU - Da Silva, Gabriela H.
AU - Franqui, Lidiane Silva
AU - De Farias, Marcelo A.
AU - De Castro, Vera Lucia S.S.
AU - Byrne, Hugh J.
AU - Martinez, Diego S.T.
AU - Monteiro, Regina T.R.
AU - Casey, Alan
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/4
Y1 - 2023/4
N2 - Titanium dioxide nanoparticles-multiwalled carbon nanotubes (TiO2−MWCNT) nanohydrid has an enhanced photocatalytic activity across the visible light with promising applications in environmental remediation, solar energy devices and antimicrobial technologies. However, it is necessary to evaluate the toxicological effects of TiO2−MWCNT towards safe and sustainable development of nanohybrids. In this work, we studied the cytotoxicity, protein corona formation and cellular internalisation of TiO2−MWCNT on fibroblasts derived from gonadal rainbow trout tissue (RTG-2) for the first time. This nanohydrid did not show any toxicity effect on RTG-2 cells up to 100 mg L−1 after 24 h of exposure as monitored by alamar blue, neutral red and trypan blue assays (in presence or absence of foetal bovine serum, FBS). Futhermore, cryo-transmission electron microscopy analysis demonstrated that TiO2 particles is attached on nanotube surface after FBS-protein corona formation in cell culture medium. Raman spectroscopy imaging showed that TiO2−MWCNT can be internalised by RTG-2 cells. This work is a novel contribution towards better understanding the nanobiointeractions of nanohydrids linked to their in vitro effects on fish cells in aquatic nanoecotoxicology.
AB - Titanium dioxide nanoparticles-multiwalled carbon nanotubes (TiO2−MWCNT) nanohydrid has an enhanced photocatalytic activity across the visible light with promising applications in environmental remediation, solar energy devices and antimicrobial technologies. However, it is necessary to evaluate the toxicological effects of TiO2−MWCNT towards safe and sustainable development of nanohybrids. In this work, we studied the cytotoxicity, protein corona formation and cellular internalisation of TiO2−MWCNT on fibroblasts derived from gonadal rainbow trout tissue (RTG-2) for the first time. This nanohydrid did not show any toxicity effect on RTG-2 cells up to 100 mg L−1 after 24 h of exposure as monitored by alamar blue, neutral red and trypan blue assays (in presence or absence of foetal bovine serum, FBS). Futhermore, cryo-transmission electron microscopy analysis demonstrated that TiO2 particles is attached on nanotube surface after FBS-protein corona formation in cell culture medium. Raman spectroscopy imaging showed that TiO2−MWCNT can be internalised by RTG-2 cells. This work is a novel contribution towards better understanding the nanobiointeractions of nanohydrids linked to their in vitro effects on fish cells in aquatic nanoecotoxicology.
KW - Ecotoxicity
KW - Nanosafety
KW - Protein corona
KW - Raman
UR - http://www.scopus.com/inward/record.url?scp=85149219014&partnerID=8YFLogxK
U2 - 10.1016/j.aquatox.2023.106434
DO - 10.1016/j.aquatox.2023.106434
M3 - Article
C2 - 36870176
AN - SCOPUS:85149219014
SN - 0166-445X
VL - 257
JO - Aquatic Toxicology
JF - Aquatic Toxicology
M1 - 106434
ER -