Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination

Y. P. Rakovich; L. Yang; C. M. Taylor; L. A. Dunbar; A. Mac Raighne; J. F. Donegan; E. M. McCabe

doi:10.1117/12.604380

Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination

Y. P. Rakovich
, L. Yang
, C. M. Taylor
, L. A. Dunbar
, A. Mac Raighne
, J. F. Donegan
, E. M. McCabe

Research output: Contribution to journal › Conference article › peer-review

Abstract

In this paper, we experimentally studied both the bright-field and fluorescence images of microspheres by conventional and confocal scanning polarization microscopes. A qualitative analysis have been given to show a physical picture on the imaging of the microspheres. Emission spectra from melainine formaldehyde microspheres stained with Ethidium Bromide or covered by thin shell of CdTe nanocrystals have been experimentally studied. We adopted analytical expressions describing the resonance spacing in order to determine the size of the microspheres.

Original language	English
Article number	31
Pages (from-to)	299-304
Number of pages	6
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5827
DOIs	https://doi.org/10.1117/12.604380
Publication status	Published - 2005
Externally published	Yes
Event	Opto-Ireland 2005: Photonic Engineering - Dublin, Ireland Duration: 4 Apr 2005 → 6 Apr 2005

Keywords

Scattering particles
Three-dimensional spectroscopy
Whispering gallery modes

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10.1117/12.604380

Cite this

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title = "Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination",

abstract = "In this paper, we experimentally studied both the bright-field and fluorescence images of microspheres by conventional and confocal scanning polarization microscopes. A qualitative analysis have been given to show a physical picture on the imaging of the microspheres. Emission spectra from melainine formaldehyde microspheres stained with Ethidium Bromide or covered by thin shell of CdTe nanocrystals have been experimentally studied. We adopted analytical expressions describing the resonance spacing in order to determine the size of the microspheres.",

keywords = "Scattering particles, Three-dimensional spectroscopy, Whispering gallery modes",

author = "Rakovich, \{Y. P.\} and L. Yang and Taylor, \{C. M.\} and Dunbar, \{L. A.\} and \{Mac Raighne\}, A. and Donegan, \{J. F.\} and McCabe, \{E. M.\}",

year = "2005",

doi = "10.1117/12.604380",

language = "English",

volume = "5827",

pages = "299--304",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Opto-Ireland 2005: Photonic Engineering ; Conference date: 04-04-2005 Through 06-04-2005",

}

TY - JOUR

T1 - Three dimensional confocal microscopy of fluorescent microspheres

T2 - Opto-Ireland 2005: Photonic Engineering

AU - Rakovich, Y. P.

AU - Yang, L.

AU - Taylor, C. M.

AU - Dunbar, L. A.

AU - Mac Raighne, A.

AU - Donegan, J. F.

AU - McCabe, E. M.

PY - 2005

Y1 - 2005

N2 - In this paper, we experimentally studied both the bright-field and fluorescence images of microspheres by conventional and confocal scanning polarization microscopes. A qualitative analysis have been given to show a physical picture on the imaging of the microspheres. Emission spectra from melainine formaldehyde microspheres stained with Ethidium Bromide or covered by thin shell of CdTe nanocrystals have been experimentally studied. We adopted analytical expressions describing the resonance spacing in order to determine the size of the microspheres.

AB - In this paper, we experimentally studied both the bright-field and fluorescence images of microspheres by conventional and confocal scanning polarization microscopes. A qualitative analysis have been given to show a physical picture on the imaging of the microspheres. Emission spectra from melainine formaldehyde microspheres stained with Ethidium Bromide or covered by thin shell of CdTe nanocrystals have been experimentally studied. We adopted analytical expressions describing the resonance spacing in order to determine the size of the microspheres.

KW - Scattering particles

KW - Three-dimensional spectroscopy

KW - Whispering gallery modes

UR - https://www.scopus.com/pages/publications/27744584346

U2 - 10.1117/12.604380

DO - 10.1117/12.604380

M3 - Conference article

AN - SCOPUS:27744584346

SN - 0277-786X

VL - 5827

SP - 299

EP - 304

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

M1 - 31

Y2 - 4 April 2005 through 6 April 2005

ER -

Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination

Abstract

Keywords

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