Solvent stable microbial lipases: current understanding and biotechnological applications

Priyanka Priyanka; Yeqi Tan; Gemma K. Kinsella; Gary T. Henehan; Barry J. Ryan

doi:10.1007/s10529-018-02633-7

Solvent stable microbial lipases: current understanding and biotechnological applications

Priyanka Priyanka, Yeqi Tan, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan

Research output: Contribution to journal › Review article › peer-review

Abstract

Objective: This review examines on our current understanding of microbial lipase solvent tolerance, with a specific focus on the molecular strategies employed to improve lipase stability in a non-aqueous environment. Results: It provides an overview of known solvent tolerant lipases and of approaches to improving solvent stability such as; enhancing stabilising interactions, modification of residue flexibility and surface charge alteration. It shows that judicious selection of lipase source supplemented by appropriate enzyme stabilisation, can lead to a wide application spectrum for lipases. Conclusion: Organic solvent stable lipases are, and will continue to be, versatile and adaptable biocatalytic workhorses commonly employed for industrial applications in the food, pharmaceutical and green manufacturing industries.

Original language	English
Pages (from-to)	203-220
Number of pages	18
Journal	Biotechnology Letters
Volume	41
Issue number	2
DOIs	https://doi.org/10.1007/s10529-018-02633-7
Publication status	Published - 15 Feb 2019

Keywords

Industrial biocatalysis
Lipase
Lipase engineering
Organic solvent stability
Organic synthesis

Access to Document

10.1007/s10529-018-02633-7

https://arrow.tudublin.ie/schfsehart/309Licence: CC BY-NC-SA

Cite this

@article{cd54aff123df48fe946e7580e17ddc94,

title = "Solvent stable microbial lipases: current understanding and biotechnological applications",

abstract = "Objective: This review examines on our current understanding of microbial lipase solvent tolerance, with a specific focus on the molecular strategies employed to improve lipase stability in a non-aqueous environment. Results: It provides an overview of known solvent tolerant lipases and of approaches to improving solvent stability such as; enhancing stabilising interactions, modification of residue flexibility and surface charge alteration. It shows that judicious selection of lipase source supplemented by appropriate enzyme stabilisation, can lead to a wide application spectrum for lipases. Conclusion: Organic solvent stable lipases are, and will continue to be, versatile and adaptable biocatalytic workhorses commonly employed for industrial applications in the food, pharmaceutical and green manufacturing industries.",

keywords = "Industrial biocatalysis, Lipase, Lipase engineering, Organic solvent stability, Organic synthesis",

author = "Priyanka Priyanka and Yeqi Tan and Kinsella, {Gemma K.} and Henehan, {Gary T.} and Ryan, {Barry J.}",

note = "Publisher Copyright: {\textcopyright} 2018, Springer Media B.V.",

year = "2019",

month = feb,

day = "15",

doi = "10.1007/s10529-018-02633-7",

language = "English",

volume = "41",

pages = "203--220",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "2",

}

TY - JOUR

T1 - Solvent stable microbial lipases

T2 - current understanding and biotechnological applications

AU - Priyanka, Priyanka

AU - Tan, Yeqi

AU - Kinsella, Gemma K.

AU - Henehan, Gary T.

AU - Ryan, Barry J.

PY - 2019/2/15

Y1 - 2019/2/15

N2 - Objective: This review examines on our current understanding of microbial lipase solvent tolerance, with a specific focus on the molecular strategies employed to improve lipase stability in a non-aqueous environment. Results: It provides an overview of known solvent tolerant lipases and of approaches to improving solvent stability such as; enhancing stabilising interactions, modification of residue flexibility and surface charge alteration. It shows that judicious selection of lipase source supplemented by appropriate enzyme stabilisation, can lead to a wide application spectrum for lipases. Conclusion: Organic solvent stable lipases are, and will continue to be, versatile and adaptable biocatalytic workhorses commonly employed for industrial applications in the food, pharmaceutical and green manufacturing industries.

AB - Objective: This review examines on our current understanding of microbial lipase solvent tolerance, with a specific focus on the molecular strategies employed to improve lipase stability in a non-aqueous environment. Results: It provides an overview of known solvent tolerant lipases and of approaches to improving solvent stability such as; enhancing stabilising interactions, modification of residue flexibility and surface charge alteration. It shows that judicious selection of lipase source supplemented by appropriate enzyme stabilisation, can lead to a wide application spectrum for lipases. Conclusion: Organic solvent stable lipases are, and will continue to be, versatile and adaptable biocatalytic workhorses commonly employed for industrial applications in the food, pharmaceutical and green manufacturing industries.

KW - Industrial biocatalysis

KW - Lipase

KW - Lipase engineering

KW - Organic solvent stability

KW - Organic synthesis

UR - http://www.scopus.com/inward/record.url?scp=85058102830&partnerID=8YFLogxK

U2 - 10.1007/s10529-018-02633-7

DO - 10.1007/s10529-018-02633-7

M3 - Review article

C2 - 30535639

AN - SCOPUS:85058102830

SN - 0141-5492

VL - 41

SP - 203

EP - 220

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 2

ER -

Solvent stable microbial lipases: current understanding and biotechnological applications

Abstract

Keywords

Access to Document

Fingerprint

Cite this