@inproceedings{2985907f248e4ea387ec26c22adac532,
title = "Self assembled nanostructures of triblock co-polymer and plasmid DNA for gene delivery",
abstract = "The formation of the self-assembled nanostructures of tri-block copolymer (PEO-PPO-PEO or TBCP) and plasmid DNA (pDNA) and their function as a non-viral vector to deliver pDNA is shown in the present work. Green fluorescence protein (GFP) pDNA was used as a model DNA. Addition of pDNA to the aqueous solution of TBCP, led to the formation of self assembled nanostructures of pDNA/TBCP. Encapsulation of DNA within the polymer micelles was proved by the gel electrophoresis experiments wherein pDNA/polymer composite didn't show any mobility under the electric field. Mixtures of different weight ratios of pDNA and TBCP have been made to screen the optimum ratio to achieve high transformation. We observed upto 6 folds higher transformation with the use of TBCP at the weight ratio of 1:10. Further, we demonstrated the integrity of the pDNA in the complex of pDNA/TBCP and the function of the pDNA in the cellular environment using E. coli DH 5α as a model microorganism.",
keywords = "Non-viral vector, Plasmid DNA, Self assembled complex, Transformation, Tri-block copolymer",
author = "Daima, {H. K.} and S. Shankar and Selvakannan, {P. R.} and Bhargava, {S. K.} and V. Bansal",
year = "2012",
language = "English",
isbn = "9781466562769",
series = "Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012",
pages = "178--181",
booktitle = "Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012",
note = "Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012 ; Conference date: 18-06-2012 Through 21-06-2012",
}