Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells

Paul Bassan; Hugh J. Byrne; Joe Lee; Franck Bonnier; Colin Clarke; Paul Dumas; Ehsan Gazi; Michael D. Brown; Noel W. Clarke; Peter Gardner

doi:10.1039/b821349f

Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells

Paul Bassan, Hugh J. Byrne, Joe Lee, Franck Bonnier, Colin Clarke, Paul Dumas, Ehsan Gazi, Michael D. Brown, Noel W. Clarke, Peter Gardner

Research output: Contribution to journal › Article › peer-review

Abstract

Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being an artefact, the reflection contribution is ever present in transflection spectra and it is further demonstrated that the reflection characteristics can be used for cellular mapping.

Original language	English
Pages (from-to)	1171-1175
Number of pages	5
Journal	Analyst
Volume	134
Issue number	6
DOIs	https://doi.org/10.1039/b821349f
Publication status	Published - 2009

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title = "Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells",

abstract = "Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being an artefact, the reflection contribution is ever present in transflection spectra and it is further demonstrated that the reflection characteristics can be used for cellular mapping.",

author = "Paul Bassan and Byrne, {Hugh J.} and Joe Lee and Franck Bonnier and Colin Clarke and Paul Dumas and Ehsan Gazi and Brown, {Michael D.} and Clarke, {Noel W.} and Peter Gardner",

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T1 - Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells

AU - Bassan, Paul

AU - Byrne, Hugh J.

AU - Lee, Joe

AU - Bonnier, Franck

AU - Clarke, Colin

AU - Dumas, Paul

AU - Gazi, Ehsan

AU - Brown, Michael D.

AU - Clarke, Noel W.

AU - Gardner, Peter

PY - 2009

Y1 - 2009

N2 - Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being an artefact, the reflection contribution is ever present in transflection spectra and it is further demonstrated that the reflection characteristics can be used for cellular mapping.

AB - Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being an artefact, the reflection contribution is ever present in transflection spectra and it is further demonstrated that the reflection characteristics can be used for cellular mapping.

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VL - 134

SP - 1171

EP - 1175

JO - Analyst

JF - Analyst

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ER -

Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells

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