TY - JOUR
T1 - Raman microspectroscopy for the early detection of pre-malignant changes in cervical tissue
AU - Rashid, Nosheen
AU - Nawaz, Haq
AU - Poon, Kelvin W.C.
AU - Bonnier, Franck
AU - Bakhiet, Salih
AU - Martin, Cara
AU - O'Leary, John J.
AU - Byrne, Hugh J.
AU - Lyng, Fiona M.
N1 - Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Cervical cancer is the third most common cancer affecting women worldwide. The mortality associated with cervical cancer can, however, be significantly reduced if the disease is detected at the pre-malignant stage. The aim of this study was to evaluate the potential of Raman microspectroscopy for elucidation of the biochemical changes associated with the pre-malignant stages of cervical cancer. Formalin fixed paraffin preserved tissue sections from cervical biopsies classified as negative for intraepithelial lesion and malignancy (NILM), low grade squamous intraepithelial lesion (LSIL) or high grade squamous intraepithelial lesion (HSIL) were analysed by Raman spectral mapping. Raman mapping, with K-means cluster analysis (KMCA), was able to differentiate the NILM cervical tissue into three layers including stroma, basal/para-basal and superficial layers, characterised by spectral features of collagen, DNA bases and glycogen respectively. In the LSIL and HSIL samples, KMCA clustered regions of the superficial layer with the basal layer. Using principal components analysis (PCA), biochemical changes associated with disease were also observed in normal areas of the abnormal samples, where morphological changes were not apparent. This study has shown that Raman microspectroscopy could be useful for the early detection of pre-malignant changes in cervical tissue.
AB - Cervical cancer is the third most common cancer affecting women worldwide. The mortality associated with cervical cancer can, however, be significantly reduced if the disease is detected at the pre-malignant stage. The aim of this study was to evaluate the potential of Raman microspectroscopy for elucidation of the biochemical changes associated with the pre-malignant stages of cervical cancer. Formalin fixed paraffin preserved tissue sections from cervical biopsies classified as negative for intraepithelial lesion and malignancy (NILM), low grade squamous intraepithelial lesion (LSIL) or high grade squamous intraepithelial lesion (HSIL) were analysed by Raman spectral mapping. Raman mapping, with K-means cluster analysis (KMCA), was able to differentiate the NILM cervical tissue into three layers including stroma, basal/para-basal and superficial layers, characterised by spectral features of collagen, DNA bases and glycogen respectively. In the LSIL and HSIL samples, KMCA clustered regions of the superficial layer with the basal layer. Using principal components analysis (PCA), biochemical changes associated with disease were also observed in normal areas of the abnormal samples, where morphological changes were not apparent. This study has shown that Raman microspectroscopy could be useful for the early detection of pre-malignant changes in cervical tissue.
KW - Cervical cancer
KW - Cervical intraepithelial neoplasia
KW - Confocal Raman microspectroscopy
KW - K-means cluster analysis
KW - Principal components analysis
UR - http://www.scopus.com/inward/record.url?scp=84910002345&partnerID=8YFLogxK
U2 - 10.1016/j.yexmp.2014.10.013
DO - 10.1016/j.yexmp.2014.10.013
M3 - Article
C2 - 25445502
AN - SCOPUS:84910002345
SN - 0014-4800
VL - 97
SP - 554
EP - 564
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 3
ER -