Abstract
Novel and sensitive plate-based fluorogenic anti-factor Xa (FXa) assays were investigated to quantify unfractionated heparin (UFH) in human plasma and whole blood within the therapeutic ranges of 0-1.6. U/mL and 0-0.8. U/mL, respectively. Two fluorogenic anti-FXa assay methods were defined for low (0-0.6. U/mL) and high (0.6-1.2. U/mL) pharmacologically relevant UFH concentration ranges in pooled human plasma. In both cases significant differences were observed at intervals of 0.2. U/mL (P<0.05). The semi-logarithmic plots of the calibration curves in the low and high UFH range were both fitted to linear regressions with correlation coefficients of 0.96 and >0.99, respectively. The assay was also optimized for whole blood which was capable of differentiating UFH concentrations at intervals of 0.2. U/mL (P<0.05) in the range of 0-0.4. U/mL. The statistically different results were fitted to a linear regression with a correlation coefficient of >0.99. The results obtained in this study could assist diagnostic laboratories towards improved monitoring of UFH therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 992-997 |
| Number of pages | 6 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 56 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 15 Dec 2011 |
| Externally published | Yes |
Keywords
- Fluorogenic anti-FXa assay
- Monitoring
- Plasma
- UFH
- Whole blood