Probing autophagy in oral cancer cells utilizing Raman spectroscopy

Isha Behl; Fiona M. Lyng; Hugh J. Byrne; Jeff O'Sullivan

doi:10.1117/12.3077508

Probing autophagy in oral cancer cells utilizing Raman spectroscopy

Isha Behl
, Fiona M. Lyng
, Hugh J. Byrne
, Jeff O'Sullivan

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This study explores the role of autophagy in oral cancer initiation and lipid metabolism using Raman microspectroscopy. Human oral carcinoma (Ca9-22) cells were starved in EBSS for 0, 4, and 8 hours to induce autophagy. Raman spectra were collected from nuclei and cytoplasm, revealing significant biochemical changes in proteins, lipids, and DNA. Principal component-linear discriminant analysis achieved discrimination accuracies of % across time points. Confocal microscopy supported autophagy initiation, showing increased acidic vesicles. Findings suggest Raman microspectroscopy as a sensitive tool for detecting autophagy in cancer cells, with future work planned to inhibit specific autophagic stages.

Original language	English
Title of host publication	Optical Biopsy XXIV
Subtitle of host publication	Toward Real-Time Spectroscopic Imaging and Diagnosis
Editors	Robert R. Alfano, Angela B. Seddon, Lingyan Shi, Binlin Wu
Publisher	SPIE
ISBN (Electronic)	9781510695993
DOIs	https://doi.org/10.1117/12.3077508
Publication status	Published - 6 Mar 2026
Event	24th Optical Biopsy: Toward Real-Time Spectroscopic Imaging and Diagnosis - San Francisco, United States Duration: 19 Jan 2026 → 21 Jan 2026

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	13843
ISSN (Print)	1605-7422
ISSN (Electronic)	2410-9045

Conference

Conference	24th Optical Biopsy: Toward Real-Time Spectroscopic Imaging and Diagnosis
Country/Territory	United States
City	San Francisco
Period	19/01/26 → 21/01/26

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1117/12.3077508Licence: Unspecified

Cite this

Behl, I., Lyng, F. M., Byrne, H. J., & O'Sullivan, J. (2026). Probing autophagy in oral cancer cells utilizing Raman spectroscopy. In R. R. Alfano, A. B. Seddon, L. Shi, & B. Wu (Eds.), Optical Biopsy XXIV: Toward Real-Time Spectroscopic Imaging and Diagnosis Article 1384304 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 13843). SPIE. https://doi.org/10.1117/12.3077508

@inproceedings{949ed26b505540bfabfe016ec4e654ac,

title = "Probing autophagy in oral cancer cells utilizing Raman spectroscopy",

abstract = "This study explores the role of autophagy in oral cancer initiation and lipid metabolism using Raman microspectroscopy. Human oral carcinoma (Ca9-22) cells were starved in EBSS for 0, 4, and 8 hours to induce autophagy. Raman spectra were collected from nuclei and cytoplasm, revealing significant biochemical changes in proteins, lipids, and DNA. Principal component-linear discriminant analysis achieved discrimination accuracies of \% across time points. Confocal microscopy supported autophagy initiation, showing increased acidic vesicles. Findings suggest Raman microspectroscopy as a sensitive tool for detecting autophagy in cancer cells, with future work planned to inhibit specific autophagic stages.",

author = "Isha Behl and Lyng, \{Fiona M.\} and Byrne, \{Hugh J.\} and Jeff O'Sullivan",

note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; 24th Optical Biopsy: Toward Real-Time Spectroscopic Imaging and Diagnosis ; Conference date: 19-01-2026 Through 21-01-2026",

year = "2026",

month = mar,

day = "6",

doi = "10.1117/12.3077508",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Alfano, \{Robert R.\} and Seddon, \{Angela B.\} and Lingyan Shi and Binlin Wu",

booktitle = "Optical Biopsy XXIV",

address = "United States",

}

Behl, I , Lyng, FM , Byrne, HJ & O'Sullivan, J 2026, Probing autophagy in oral cancer cells utilizing Raman spectroscopy. in RR Alfano, AB Seddon, L Shi & B Wu (eds), Optical Biopsy XXIV: Toward Real-Time Spectroscopic Imaging and Diagnosis., 1384304, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 13843, SPIE, 24th Optical Biopsy: Toward Real-Time Spectroscopic Imaging and Diagnosis, San Francisco, United States, 19/01/26. https://doi.org/10.1117/12.3077508

Probing autophagy in oral cancer cells utilizing Raman spectroscopy. / Behl, Isha ; Lyng, Fiona M.; Byrne, Hugh J. et al.
Optical Biopsy XXIV: Toward Real-Time Spectroscopic Imaging and Diagnosis. ed. / Robert R. Alfano; Angela B. Seddon; Lingyan Shi; Binlin Wu. SPIE, 2026. 1384304 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 13843).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Probing autophagy in oral cancer cells utilizing Raman spectroscopy

AU - Behl, Isha

AU - Lyng, Fiona M.

AU - Byrne, Hugh J.

AU - O'Sullivan, Jeff

PY - 2026/3/6

Y1 - 2026/3/6

N2 - This study explores the role of autophagy in oral cancer initiation and lipid metabolism using Raman microspectroscopy. Human oral carcinoma (Ca9-22) cells were starved in EBSS for 0, 4, and 8 hours to induce autophagy. Raman spectra were collected from nuclei and cytoplasm, revealing significant biochemical changes in proteins, lipids, and DNA. Principal component-linear discriminant analysis achieved discrimination accuracies of % across time points. Confocal microscopy supported autophagy initiation, showing increased acidic vesicles. Findings suggest Raman microspectroscopy as a sensitive tool for detecting autophagy in cancer cells, with future work planned to inhibit specific autophagic stages.

AB - This study explores the role of autophagy in oral cancer initiation and lipid metabolism using Raman microspectroscopy. Human oral carcinoma (Ca9-22) cells were starved in EBSS for 0, 4, and 8 hours to induce autophagy. Raman spectra were collected from nuclei and cytoplasm, revealing significant biochemical changes in proteins, lipids, and DNA. Principal component-linear discriminant analysis achieved discrimination accuracies of % across time points. Confocal microscopy supported autophagy initiation, showing increased acidic vesicles. Findings suggest Raman microspectroscopy as a sensitive tool for detecting autophagy in cancer cells, with future work planned to inhibit specific autophagic stages.

UR - https://www.scopus.com/pages/publications/105035626703

U2 - 10.1117/12.3077508

DO - 10.1117/12.3077508

M3 - Conference contribution

AN - SCOPUS:105035626703

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Optical Biopsy XXIV

A2 - Alfano, Robert R.

A2 - Seddon, Angela B.

A2 - Shi, Lingyan

A2 - Wu, Binlin

PB - SPIE

T2 - 24th Optical Biopsy: Toward Real-Time Spectroscopic Imaging and Diagnosis

Y2 - 19 January 2026 through 21 January 2026

ER -

Probing autophagy in oral cancer cells utilizing Raman spectroscopy

Abstract

Publication series

Conference

UN SDGs

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