Potential Microbial Consortium Mitigates Drought Stress in Tomato (Solanum lycopersicum L.) Plant by Up-regulating Stress-Responsive Genes and Improving Fruit Yield and Soil Properties

Ram Krishna; Durgesh Kumar Jaiswal; Waquar Akhter Ansari; Saurabh Singh; PS Soumia; Achuit Kumar Singh; Babita Kumari; Major Singh; Jay Prakash Verma

Potential Microbial Consortium Mitigates Drought Stress in Tomato (Solanum lycopersicum L.) Plant by Up-regulating Stress-Responsive Genes and Improving Fruit Yield and Soil Properties

Ram Krishna
, Durgesh Kumar Jaiswal
, Waquar Akhter Ansari
, Saurabh Singh
, PS Soumia
, Achuit Kumar Singh
, Babita Kumari
, Major Singh
, Jay Prakash Verma

School of Chemical and BioPharmaceutical Science

Research output: Contribution to journal › Article › peer-review

Abstract

The present study is conducted for the growth and yield improvement of tomato plants under drought stress by inoculation of hexa plant growth-promoting microorganisms (PGPM). Hexa-PGPM consortium (Bacillus megaterium, Pseudomonas fluorescens, P. aeruginosa, P. putida, Paenibacillus polymyxa, and Trichoderma harzianum) is inoculated to the tomato plant, and growth attributes, membrane integrity, water status, accumulation of osmolyte, reactive oxygen species (ROS) scavenging capability, and the qRT-PCR analysis were performed for expression of stress-responsive DREB, APX, CAT, SOD, and P ₅CS under 80% and 40% moisture content of the field capacity. Soil physico-chemical and microbial properties were also evaluated. Our results revealed that under drought, hexa-PGPM consortium-inoculated plants exhibited lower cellular damage and better plant growth and yield than non-inoculated plants. Antioxidant enzyme catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX) activity decreases under drought stress condition and it increases in hexa-PGPM-inoculated plants. Simultaneously, the gene expression analysis showed up-regulation of a transcriptional activator (DREB1), osmolyte accumulators (P ₅CS), and ROS scavengers (CAT, SOD, APX) gene by application of hexa-PGPM consortium. Overall, the results showed that the hexa-PGPM application confers drought mitigation in tomatoes by altering different physico-biochemical and molecular parameters. In addition, the PGPM application also improved the soil’s physical, chemico-chemical, and biological properties under drought stress conditions. The present study supports the application of hexa- PGPM consortium to elevate drought tolerance, yield, and soil fertility enhancement under drought stress as a low-cost agro-biotechnology tool and environment-friendly drought management techniques in tomato crops.

Original language	English
Pages (from-to)	4598-4615
Number of pages	18
Journal	Journal of Soil Science and Plant Nutrition
Volume	22
Issue number	4
Publication status	Published - Dec 2022

Keywords

Antioxidant enzyme
Drought
Microbial consortia
Plant growth and production
Relative expression
Solanum lycopersicum

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https://link.springer.com/article/10.1007/s42729-022-00929-2Licence: Unspecified

Cite this

Krishna, R., Jaiswal, D. K., Ansari, W. A., Singh, S., Soumia, PS., Singh, A. K., Kumari, B., Singh, M., & Verma, J. P. (2022). Potential Microbial Consortium Mitigates Drought Stress in Tomato (Solanum lycopersicum L.) Plant by Up-regulating Stress-Responsive Genes and Improving Fruit Yield and Soil Properties. Journal of Soil Science and Plant Nutrition, 22(4), 4598-4615. https://link.springer.com/article/10.1007/s42729-022-00929-2

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title = "Potential Microbial Consortium Mitigates Drought Stress in Tomato (Solanum lycopersicum L.) Plant by Up-regulating Stress-Responsive Genes and Improving Fruit Yield and Soil Properties",

abstract = "The present study is conducted for the growth and yield improvement of tomato plants under drought stress by inoculation of hexa plant growth-promoting microorganisms (PGPM). Hexa-PGPM consortium (Bacillus megaterium, Pseudomonas fluorescens, P. aeruginosa, P. putida, Paenibacillus polymyxa, and Trichoderma harzianum) is inoculated to the tomato plant, and growth attributes, membrane integrity, water status, accumulation of osmolyte, reactive oxygen species (ROS) scavenging capability, and the qRT-PCR analysis were performed for expression of stress-responsive DREB, APX, CAT, SOD, and P 5CS under 80\% and 40\% moisture content of the field capacity. Soil physico-chemical and microbial properties were also evaluated. Our results revealed that under drought, hexa-PGPM consortium-inoculated plants exhibited lower cellular damage and better plant growth and yield than non-inoculated plants. Antioxidant enzyme catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX) activity decreases under drought stress condition and it increases in hexa-PGPM-inoculated plants. Simultaneously, the gene expression analysis showed up-regulation of a transcriptional activator (DREB1), osmolyte accumulators (P 5CS), and ROS scavengers (CAT, SOD, APX) gene by application of hexa-PGPM consortium. Overall, the results showed that the hexa-PGPM application confers drought mitigation in tomatoes by altering different physico-biochemical and molecular parameters. In addition, the PGPM application also improved the soil{\textquoteright}s physical, chemico-chemical, and biological properties under drought stress conditions. The present study supports the application of hexa- PGPM consortium to elevate drought tolerance, yield, and soil fertility enhancement under drought stress as a low-cost agro-biotechnology tool and environment-friendly drought management techniques in tomato crops.",

keywords = "Antioxidant enzyme, Drought, Microbial consortia, Plant growth and production, Relative expression, Solanum lycopersicum",

author = "Ram Krishna and Jaiswal, \{Durgesh Kumar\} and Ansari, \{Waquar Akhter\} and Saurabh Singh and PS Soumia and Singh, \{Achuit Kumar\} and Babita Kumari and Major Singh and Verma, \{Jay Prakash\}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo.",

year = "2022",

month = dec,

language = "English",

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pages = "4598--4615",

journal = "Journal of Soil Science and Plant Nutrition",

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Krishna, R, Jaiswal, DK, Ansari, WA, Singh, S, Soumia, PS, Singh, AK, Kumari, B, Singh, M & Verma, JP 2022, 'Potential Microbial Consortium Mitigates Drought Stress in Tomato (Solanum lycopersicum L.) Plant by Up-regulating Stress-Responsive Genes and Improving Fruit Yield and Soil Properties', Journal of Soil Science and Plant Nutrition, vol. 22, no. 4, pp. 4598-4615. <https://link.springer.com/article/10.1007/s42729-022-00929-2>

Potential Microbial Consortium Mitigates Drought Stress in Tomato (Solanum lycopersicum L.) Plant by Up-regulating Stress-Responsive Genes and Improving Fruit Yield and Soil Properties. / Krishna, Ram; Jaiswal, Durgesh Kumar; Ansari, Waquar Akhter et al.
In: Journal of Soil Science and Plant Nutrition, Vol. 22, No. 4, 12.2022, p. 4598-4615.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Potential Microbial Consortium Mitigates Drought Stress in Tomato (Solanum lycopersicum L.) Plant by Up-regulating Stress-Responsive Genes and Improving Fruit Yield and Soil Properties

AU - Krishna, Ram

AU - Jaiswal, Durgesh Kumar

AU - Ansari, Waquar Akhter

AU - Singh, Saurabh

AU - Soumia, PS

AU - Singh, Achuit Kumar

AU - Kumari, Babita

AU - Singh, Major

AU - Verma, Jay Prakash

PY - 2022/12

Y1 - 2022/12

N2 - The present study is conducted for the growth and yield improvement of tomato plants under drought stress by inoculation of hexa plant growth-promoting microorganisms (PGPM). Hexa-PGPM consortium (Bacillus megaterium, Pseudomonas fluorescens, P. aeruginosa, P. putida, Paenibacillus polymyxa, and Trichoderma harzianum) is inoculated to the tomato plant, and growth attributes, membrane integrity, water status, accumulation of osmolyte, reactive oxygen species (ROS) scavenging capability, and the qRT-PCR analysis were performed for expression of stress-responsive DREB, APX, CAT, SOD, and P 5CS under 80% and 40% moisture content of the field capacity. Soil physico-chemical and microbial properties were also evaluated. Our results revealed that under drought, hexa-PGPM consortium-inoculated plants exhibited lower cellular damage and better plant growth and yield than non-inoculated plants. Antioxidant enzyme catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX) activity decreases under drought stress condition and it increases in hexa-PGPM-inoculated plants. Simultaneously, the gene expression analysis showed up-regulation of a transcriptional activator (DREB1), osmolyte accumulators (P 5CS), and ROS scavengers (CAT, SOD, APX) gene by application of hexa-PGPM consortium. Overall, the results showed that the hexa-PGPM application confers drought mitigation in tomatoes by altering different physico-biochemical and molecular parameters. In addition, the PGPM application also improved the soil’s physical, chemico-chemical, and biological properties under drought stress conditions. The present study supports the application of hexa- PGPM consortium to elevate drought tolerance, yield, and soil fertility enhancement under drought stress as a low-cost agro-biotechnology tool and environment-friendly drought management techniques in tomato crops.

AB - The present study is conducted for the growth and yield improvement of tomato plants under drought stress by inoculation of hexa plant growth-promoting microorganisms (PGPM). Hexa-PGPM consortium (Bacillus megaterium, Pseudomonas fluorescens, P. aeruginosa, P. putida, Paenibacillus polymyxa, and Trichoderma harzianum) is inoculated to the tomato plant, and growth attributes, membrane integrity, water status, accumulation of osmolyte, reactive oxygen species (ROS) scavenging capability, and the qRT-PCR analysis were performed for expression of stress-responsive DREB, APX, CAT, SOD, and P 5CS under 80% and 40% moisture content of the field capacity. Soil physico-chemical and microbial properties were also evaluated. Our results revealed that under drought, hexa-PGPM consortium-inoculated plants exhibited lower cellular damage and better plant growth and yield than non-inoculated plants. Antioxidant enzyme catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX) activity decreases under drought stress condition and it increases in hexa-PGPM-inoculated plants. Simultaneously, the gene expression analysis showed up-regulation of a transcriptional activator (DREB1), osmolyte accumulators (P 5CS), and ROS scavengers (CAT, SOD, APX) gene by application of hexa-PGPM consortium. Overall, the results showed that the hexa-PGPM application confers drought mitigation in tomatoes by altering different physico-biochemical and molecular parameters. In addition, the PGPM application also improved the soil’s physical, chemico-chemical, and biological properties under drought stress conditions. The present study supports the application of hexa- PGPM consortium to elevate drought tolerance, yield, and soil fertility enhancement under drought stress as a low-cost agro-biotechnology tool and environment-friendly drought management techniques in tomato crops.

KW - Antioxidant enzyme

KW - Drought

KW - Microbial consortia

KW - Plant growth and production

KW - Relative expression

KW - Solanum lycopersicum

UR - https://www.scopus.com/pages/publications/85137540118

M3 - Article

VL - 22

SP - 4598

EP - 4615

JO - Journal of Soil Science and Plant Nutrition

JF - Journal of Soil Science and Plant Nutrition

IS - 4

ER -

Potential Microbial Consortium Mitigates Drought Stress in Tomato (Solanum lycopersicum L.) Plant by Up-regulating Stress-Responsive Genes and Improving Fruit Yield and Soil Properties

Abstract

Keywords

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