Plasma induced reactive oxygen species-dependent cytotoxicity in glioblastoma 3D tumourspheres

Janith Wanigasekara; Carlos Barcia; Patrick J. Cullen; Brijesh Tiwari; James F. Curtin

doi:10.1002/ppap.202100157

Plasma induced reactive oxygen species-dependent cytotoxicity in glioblastoma 3D tumourspheres

Janith Wanigasekara, Carlos Barcia, Patrick J. Cullen, Brijesh Tiwari, James F. Curtin

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to determine the effects of a pin-to-plate cold atmospheric plasma (CAP) on U-251 MG three-dimensional (3D) glioblastoma spheroids under different conditions. 3D tumorspheres showed higher resistance to the CAP treatment compared to 2D monolayer cells. A single CAP treatment was able to induce cytotoxicity, while multiple CAP treatments augmented this effect. CAP was also able to induce cytotoxicity throughout the tumoursphere, and we identified that reactive oxygen species (ROS) plays a major role, while H₂O₂ plays a partial role in CAP-induced cytotoxicity in tumourspheres. We conclude that ROS-dependent cytotoxicity is induced uniformly throughout glioblastoma and epidermoid tumourspheres by direct CAP treatment.

Original language	English
Article number	2100157
Journal	Plasma Processes and Polymers
Volume	19
Issue number	4
DOIs	https://doi.org/10.1002/ppap.202100157
Publication status	Published - Apr 2022

Keywords

3D tumourspheres
ROS dependent
cold atmospheric plasma
cytotoxicity
epidermoid
glioblastoma
reactive oxygen and nitrogen species

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10.1002/ppap.202100157Licence: CC BY

https://arrow.tudublin.ie/creaart/109Licence: CC BY

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title = "Plasma induced reactive oxygen species-dependent cytotoxicity in glioblastoma 3D tumourspheres",

abstract = "The aim of this study was to determine the effects of a pin-to-plate cold atmospheric plasma (CAP) on U-251 MG three-dimensional (3D) glioblastoma spheroids under different conditions. 3D tumorspheres showed higher resistance to the CAP treatment compared to 2D monolayer cells. A single CAP treatment was able to induce cytotoxicity, while multiple CAP treatments augmented this effect. CAP was also able to induce cytotoxicity throughout the tumoursphere, and we identified that reactive oxygen species (ROS) plays a major role, while H2O2 plays a partial role in CAP-induced cytotoxicity in tumourspheres. We conclude that ROS-dependent cytotoxicity is induced uniformly throughout glioblastoma and epidermoid tumourspheres by direct CAP treatment.",

keywords = "3D tumourspheres, ROS dependent, cold atmospheric plasma, cytotoxicity, epidermoid, glioblastoma, reactive oxygen and nitrogen species",

author = "Janith Wanigasekara and Carlos Barcia and Cullen, {Patrick J.} and Brijesh Tiwari and Curtin, {James F.}",

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T1 - Plasma induced reactive oxygen species-dependent cytotoxicity in glioblastoma 3D tumourspheres

AU - Wanigasekara, Janith

AU - Barcia, Carlos

AU - Cullen, Patrick J.

AU - Tiwari, Brijesh

AU - Curtin, James F.

PY - 2022/4

Y1 - 2022/4

N2 - The aim of this study was to determine the effects of a pin-to-plate cold atmospheric plasma (CAP) on U-251 MG three-dimensional (3D) glioblastoma spheroids under different conditions. 3D tumorspheres showed higher resistance to the CAP treatment compared to 2D monolayer cells. A single CAP treatment was able to induce cytotoxicity, while multiple CAP treatments augmented this effect. CAP was also able to induce cytotoxicity throughout the tumoursphere, and we identified that reactive oxygen species (ROS) plays a major role, while H2O2 plays a partial role in CAP-induced cytotoxicity in tumourspheres. We conclude that ROS-dependent cytotoxicity is induced uniformly throughout glioblastoma and epidermoid tumourspheres by direct CAP treatment.

AB - The aim of this study was to determine the effects of a pin-to-plate cold atmospheric plasma (CAP) on U-251 MG three-dimensional (3D) glioblastoma spheroids under different conditions. 3D tumorspheres showed higher resistance to the CAP treatment compared to 2D monolayer cells. A single CAP treatment was able to induce cytotoxicity, while multiple CAP treatments augmented this effect. CAP was also able to induce cytotoxicity throughout the tumoursphere, and we identified that reactive oxygen species (ROS) plays a major role, while H2O2 plays a partial role in CAP-induced cytotoxicity in tumourspheres. We conclude that ROS-dependent cytotoxicity is induced uniformly throughout glioblastoma and epidermoid tumourspheres by direct CAP treatment.

KW - 3D tumourspheres

KW - ROS dependent

KW - cold atmospheric plasma

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KW - reactive oxygen and nitrogen species

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DO - 10.1002/ppap.202100157

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Plasma induced reactive oxygen species-dependent cytotoxicity in glioblastoma 3D tumourspheres

Abstract

Keywords

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