TY - JOUR
T1 - Optimization of ultrasound assisted extraction of antioxidant compounds from marjoram (Origanum majorana L.) using response surface methodology
AU - Hossain, Mohammad B.
AU - Brunton, Nigel P.
AU - Patras, Ankit
AU - Tiwari, Brijesh
AU - O'Donnell, C. P.
AU - Martin-Diana, Ana B.
AU - Barry-Ryan, Catherine
N1 - Funding Information:
This work was supported by the Irish Department of Agriculture, Fisheries and Food under the Food Institutional Research Measure and by the ABBEST scholarship program of Dublin Institute of Technology, Ireland. The authors would also likely to acknowledge AllinAll Ingredients Ltd. for providing spice samples.
PY - 2012/5
Y1 - 2012/5
N2 - The present study optimized the ultrasound assisted extraction (UAE) conditions to maximize the antioxidant activity [Ferric ion Reducing Antioxidant Power (FRAP)], total phenol content (TP) and content of individual polyphenols of extracts from marjoram. Optimal conditions with regard to amplitude of sonication (24.4-61.0 μm) and extraction temperature (15-35°C) and extraction time (5-15 min) were identified using response surface methodology (RSM). The results showed that the combined treatment conditions of 61 μm, 35°C and 15 min were optimal for maximizing TP, FRAP, rosmarinic acid, luteolin-7-O-glucoside, apigenin-7-O-glucoside, caffeic acid, carnosic acid and carnosol values of the extracts. The predicted values from the developed quadratic polynomial equation were in close agreement with the actual experimental values with low average mean deviations (E%) ranging from 0.45% to 1.55%. The extraction yields of the optimal UAE were significantly (p < 0.05) higher than solid/liquid extracts. Predicted models were highly significant (p < 0.05) for all the parameters studied with high regression coefficients (R2) ranging from 0.58 to 0.989.
AB - The present study optimized the ultrasound assisted extraction (UAE) conditions to maximize the antioxidant activity [Ferric ion Reducing Antioxidant Power (FRAP)], total phenol content (TP) and content of individual polyphenols of extracts from marjoram. Optimal conditions with regard to amplitude of sonication (24.4-61.0 μm) and extraction temperature (15-35°C) and extraction time (5-15 min) were identified using response surface methodology (RSM). The results showed that the combined treatment conditions of 61 μm, 35°C and 15 min were optimal for maximizing TP, FRAP, rosmarinic acid, luteolin-7-O-glucoside, apigenin-7-O-glucoside, caffeic acid, carnosic acid and carnosol values of the extracts. The predicted values from the developed quadratic polynomial equation were in close agreement with the actual experimental values with low average mean deviations (E%) ranging from 0.45% to 1.55%. The extraction yields of the optimal UAE were significantly (p < 0.05) higher than solid/liquid extracts. Predicted models were highly significant (p < 0.05) for all the parameters studied with high regression coefficients (R2) ranging from 0.58 to 0.989.
KW - Antioxidant
KW - RSM
KW - Spice
KW - Total phenols
KW - Ultrasound assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=84855261880&partnerID=8YFLogxK
U2 - 10.1016/j.ultsonch.2011.11.001
DO - 10.1016/j.ultsonch.2011.11.001
M3 - Article
C2 - 22172467
AN - SCOPUS:84855261880
SN - 1350-4177
VL - 19
SP - 582
EP - 590
JO - Ultrasonics Sonochemistry
JF - Ultrasonics Sonochemistry
IS - 3
ER -