Optimization of ultrasound assisted extraction of antioxidant compounds from marjoram (Origanum majorana L.) using response surface methodology

Mohammad B. Hossain, Nigel P. Brunton, Ankit Patras, Brijesh Tiwari, C. P. O'Donnell, Ana B. Martin-Diana, Catherine Barry-Ryan

Research output: Contribution to journalArticlepeer-review

Abstract

The present study optimized the ultrasound assisted extraction (UAE) conditions to maximize the antioxidant activity [Ferric ion Reducing Antioxidant Power (FRAP)], total phenol content (TP) and content of individual polyphenols of extracts from marjoram. Optimal conditions with regard to amplitude of sonication (24.4-61.0 μm) and extraction temperature (15-35°C) and extraction time (5-15 min) were identified using response surface methodology (RSM). The results showed that the combined treatment conditions of 61 μm, 35°C and 15 min were optimal for maximizing TP, FRAP, rosmarinic acid, luteolin-7-O-glucoside, apigenin-7-O-glucoside, caffeic acid, carnosic acid and carnosol values of the extracts. The predicted values from the developed quadratic polynomial equation were in close agreement with the actual experimental values with low average mean deviations (E%) ranging from 0.45% to 1.55%. The extraction yields of the optimal UAE were significantly (p < 0.05) higher than solid/liquid extracts. Predicted models were highly significant (p < 0.05) for all the parameters studied with high regression coefficients (R2) ranging from 0.58 to 0.989.

Original languageEnglish
Pages (from-to)582-590
Number of pages9
JournalUltrasonics Sonochemistry
Volume19
Issue number3
DOIs
Publication statusPublished - May 2012

Keywords

  • Antioxidant
  • RSM
  • Spice
  • Total phenols
  • Ultrasound assisted extraction

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