TY - JOUR
T1 - New immobilization method of anti-PepD monoclonal antibodies for the detection of Listeria monocytogenes p60 protein – Part B
T2 - Rapid and specific sandwich ELISA using antibodies immobilized on a chitosan/CNC film support
AU - Etty, Marie Christine
AU - D'Auria, Sabato
AU - Shankar, Shiv
AU - Salmieri, Stephane
AU - Fraschini, Carole
AU - Lacroix, Monique
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10
Y1 - 2019/10
N2 - This paper presents the application of a new support based on chitosan and reinforced with cellulose nanocrystals (m-CCG) for the detection of Listeria monocytogenes p60 protein in sandwich ELISA. This support consists of biopolymer membrane optimized for the covalent immobilization of the specific monoclonal antibodies against the unique short peptide of eleven amino acids (PepD, QQQTAPKAPTE) of L. monocytogenes p60 protein (anti-PepD mAb). Using the optimized immobilization method of anti-PepD mAb on m-CCG (m-CCG + anti-PepD mAb), the detection of L. monocytogenes culture was improved by 17% in Tryptic soy broth and by 24% in Listeria enrichment broth containing 0.5% of dextrose (modified LEB) on m-CCG compared to standard ELISA support. Moreover, the evaluation of the selectivity of detection of L. monocytogenes by anti-PepD mAb immobilized on m-CCG allowed the absence of any cross-reaction between the support and the nine other bacteria commonly found in food environment.
AB - This paper presents the application of a new support based on chitosan and reinforced with cellulose nanocrystals (m-CCG) for the detection of Listeria monocytogenes p60 protein in sandwich ELISA. This support consists of biopolymer membrane optimized for the covalent immobilization of the specific monoclonal antibodies against the unique short peptide of eleven amino acids (PepD, QQQTAPKAPTE) of L. monocytogenes p60 protein (anti-PepD mAb). Using the optimized immobilization method of anti-PepD mAb on m-CCG (m-CCG + anti-PepD mAb), the detection of L. monocytogenes culture was improved by 17% in Tryptic soy broth and by 24% in Listeria enrichment broth containing 0.5% of dextrose (modified LEB) on m-CCG compared to standard ELISA support. Moreover, the evaluation of the selectivity of detection of L. monocytogenes by anti-PepD mAb immobilized on m-CCG allowed the absence of any cross-reaction between the support and the nine other bacteria commonly found in food environment.
KW - Antibodies immobilization
KW - Cellulose nanocrystals
KW - Chitosan
KW - Listeria monocytogenes
KW - p60 protein
KW - Sandwich ELISA
UR - https://www.scopus.com/pages/publications/85068567641
U2 - 10.1016/j.reactfunctpolym.2019.104317
DO - 10.1016/j.reactfunctpolym.2019.104317
M3 - Article
AN - SCOPUS:85068567641
SN - 1381-5148
VL - 143
JO - Reactive and Functional Polymers
JF - Reactive and Functional Polymers
M1 - 104317
ER -