Label-free infrared spectroscopic imaging for characterization of necrotic tissue areas on cutaneous squamous cell carcinoma

Cássio A. Lima, Luciana Correa, Hugh J. Byrne, Denise M. Zezell

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this study, FTIR spectroscopy was used to evaluate the overall biochemical status of necrotic tissue areas of cutaneous squamous cell carcinoma chemically-induced on mice. FTIR hyperspectral image collected from specimen showed high correlation with the photomicrograph obtained by light microscopy, in which we were able to identify clusters associated to keratin, necrosis and regions with no tissue. Alterations in the protein content were documented in the necrotic tissue areas, indicating changes on protein conformation.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII
EditorsWarren S. Grundfest, Anita Mahadevan-Jansen
PublisherSPIE
ISBN (Electronic)9781510623781
DOIs
Publication statusPublished - 2019
EventAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII 2019 - San Francisco, United States
Duration: 3 Feb 20195 Feb 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10868
ISSN (Print)1605-7422

Conference

ConferenceAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII 2019
Country/TerritoryUnited States
CitySan Francisco
Period3/02/195/02/19

Keywords

  • Cutaneous squamous cell carcinoma
  • Death mechanisms
  • FTIR microspectroscopy
  • KMCA
  • Necrosis

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