Investigating the use of Raman and immersion Raman spectroscopy for spectral histopathology of metastatic brain cancer and primary sites of origin

Leanne M. Fullwood, Graeme Clemens, Dave Griffiths, Katherine Ashton, Timothy P. Dawson, Robert W. Lea, Charles Davis, Franck Bonnier, Hugh J. Byrne, Matthew J. Baker

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    It is estimated that approximately 13000 people in the UK are diagnosed with brain cancer every year; of which 60% are metastatic. Current methods of diagnosis can be subjective, invasive and have long diagnostic windows. Raman spectroscopy provides a non-destructive, non-invasive, rapid and economical method for diagnosing diseases. The aim of this study was to investigate the use of Raman and immersion Raman spectroscopy for diagnosing metastatic brain cancer and identifying primary sites of origin using brain tissue. Through spectral examination, the Raman peaks at 721 cm-1 and 782 cm -1 were identified as being the most distinct for discriminating between the glioblastoma multiforme (GBM), metastatic and normal brain tissue spectra. A ratio score plot of these peaks calculated the classification sensitivities and specificities as 100% and 94.44% for GBM, 96.55% and 100% for metastatic brain, and 85.71% and 100% for normal brain tissue. Principal Component-Linear Discriminant Analysis (PC-LDA) also showed discrimination between normal, GBM and metastatic brain tissue spectra. We also present, for the first time, the use of Raman spectroscopy to investigate primary site of origin for metastatic brain cancer and any biochemical differences between different primary and metastatic cancer using linked samples. This study revealed interesting spectral differences in the amide regions showing changes in the biochemistry of the metastatic brain cancer from the primary cancer.

    Original languageEnglish
    Pages (from-to)3948-3961
    Number of pages14
    JournalAnalytical Methods
    Volume6
    Issue number12
    DOIs
    Publication statusPublished - 21 Jun 2014

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