Investigating the role of shape on the biological impact of gold nanoparticles in vitro

Furong Tian; Martin J.D. Clift; Alan Casey; Pablo Del Pino; Beatriz Pelaz; João Conde; Hugh J. Byrne; Barbara Rothen-Rutishauser; Giovani Estrada; Jesús M. De La Fuente; Tobias Stoeger

doi:10.2217/nnm.15.103

Investigating the role of shape on the biological impact of gold nanoparticles in vitro

Furong Tian, Martin J.D. Clift, Alan Casey, Pablo Del Pino, Beatriz Pelaz, João Conde, Hugh J. Byrne, Barbara Rothen-Rutishauser, Giovani Estrada, Jesús M. De La Fuente, Tobias Stoeger

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. Materials & methods: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). Results: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

Original language	English
Pages (from-to)	2643-2657
Number of pages	15
Journal	Nanomedicine
Volume	10
Issue number	17
DOIs	https://doi.org/10.2217/nnm.15.103
Publication status	Published - 1 Sep 2015

Keywords

biocompatibility
caspases
Cathepsin-B
CD95 (APO-1/Fas)
cell death
gold nanoparticles
lung epithelial cells
nanoparticle shape
nanotoxicology
reactive oxygen species

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@article{7d4b9c7250c54758894a4da8beb27fa9,

title = "Investigating the role of shape on the biological impact of gold nanoparticles in vitro",

abstract = "Aim: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. Materials \& methods: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). Results: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60\%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.",

keywords = "biocompatibility, caspases, Cathepsin-B, CD95 (APO-1/Fas), cell death, gold nanoparticles, lung epithelial cells, nanoparticle shape, nanotoxicology, reactive oxygen species",

author = "Furong Tian and Clift, \{Martin J.D.\} and Alan Casey and \{Del Pino\}, Pablo and Beatriz Pelaz and Jo{\~a}o Conde and Byrne, \{Hugh J.\} and Barbara Rothen-Rutishauser and Giovani Estrada and \{De La Fuente\}, \{Jes{\'u}s M.\} and Tobias Stoeger",

note = "Publisher Copyright: {\textcopyright} 2015 Future Medicine Ltd.",

year = "2015",

month = sep,

day = "1",

doi = "10.2217/nnm.15.103",

language = "English",

volume = "10",

pages = "2643--2657",

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T1 - Investigating the role of shape on the biological impact of gold nanoparticles in vitro

AU - Tian, Furong

AU - Clift, Martin J.D.

AU - Casey, Alan

AU - Del Pino, Pablo

AU - Pelaz, Beatriz

AU - Conde, João

AU - Byrne, Hugh J.

AU - Rothen-Rutishauser, Barbara

AU - Estrada, Giovani

AU - De La Fuente, Jesús M.

AU - Stoeger, Tobias

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Aim: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. Materials & methods: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). Results: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

AB - Aim: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. Materials & methods: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). Results: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

KW - biocompatibility

KW - caspases

KW - Cathepsin-B

KW - CD95 (APO-1/Fas)

KW - cell death

KW - gold nanoparticles

KW - lung epithelial cells

KW - nanoparticle shape

KW - nanotoxicology

KW - reactive oxygen species

UR - https://www.scopus.com/pages/publications/84941886516

U2 - 10.2217/nnm.15.103

DO - 10.2217/nnm.15.103

M3 - Article

C2 - 26377045

AN - SCOPUS:84941886516

SN - 1743-5889

VL - 10

SP - 2643

EP - 2657

JO - Nanomedicine

JF - Nanomedicine

IS - 17

ER -

Investigating the role of shape on the biological impact of gold nanoparticles in vitro

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Keywords

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