Investigating the role of shape on the biological impact of gold nanoparticles in vitro

Furong Tian; Martin J.D. Clift; Alan Casey; Pablo Del Pino; Beatriz Pelaz; João Conde; Hugh J. Byrne; Barbara Rothen-Rutishauser; Giovani Estrada; Jesús M. De La Fuente; Tobias Stoeger

doi:10.2217/nnm.15.103

Investigating the role of shape on the biological impact of gold nanoparticles in vitro

Furong Tian
, Martin J.D. Clift
, Alan Casey
, Pablo Del Pino
, Beatriz Pelaz
, João Conde
, Hugh J. Byrne
, Barbara Rothen-Rutishauser
, Giovani Estrada
, Jesús M. De La Fuente
, Tobias Stoeger

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. Materials & methods: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). Results: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

Original language	English
Pages (from-to)	2643-2657
Number of pages	15
Journal	Nanomedicine
Volume	10
Issue number	17
DOIs	https://doi.org/10.2217/nnm.15.103
Publication status	Published - 1 Sep 2015

Keywords

biocompatibility
caspases
Cathepsin-B
CD95 (APO-1/Fas)
cell death
gold nanoparticles
lung epithelial cells
nanoparticle shape
nanotoxicology
reactive oxygen species

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https://arrow.tudublin.ie/biophonart/23Licence: CC BY-NC-SA

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@article{7d4b9c7250c54758894a4da8beb27fa9,

title = "Investigating the role of shape on the biological impact of gold nanoparticles in vitro",

abstract = "Aim: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. Materials \& methods: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). Results: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60\%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.",

keywords = "biocompatibility, caspases, Cathepsin-B, CD95 (APO-1/Fas), cell death, gold nanoparticles, lung epithelial cells, nanoparticle shape, nanotoxicology, reactive oxygen species",

author = "Furong Tian and Clift, \{Martin J.D.\} and Alan Casey and \{Del Pino\}, Pablo and Beatriz Pelaz and Jo{\~a}o Conde and Byrne, \{Hugh J.\} and Barbara Rothen-Rutishauser and Giovani Estrada and \{De La Fuente\}, \{Jes{\'u}s M.\} and Tobias Stoeger",

note = "Publisher Copyright: {\textcopyright} 2015 Future Medicine Ltd.",

year = "2015",

month = sep,

day = "1",

doi = "10.2217/nnm.15.103",

language = "English",

volume = "10",

pages = "2643--2657",

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T1 - Investigating the role of shape on the biological impact of gold nanoparticles in vitro

AU - Tian, Furong

AU - Clift, Martin J.D.

AU - Casey, Alan

AU - Del Pino, Pablo

AU - Pelaz, Beatriz

AU - Conde, João

AU - Byrne, Hugh J.

AU - Rothen-Rutishauser, Barbara

AU - Estrada, Giovani

AU - De La Fuente, Jesús M.

AU - Stoeger, Tobias

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Aim: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. Materials & methods: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). Results: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

AB - Aim: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. Materials & methods: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). Results: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

KW - biocompatibility

KW - caspases

KW - Cathepsin-B

KW - CD95 (APO-1/Fas)

KW - cell death

KW - gold nanoparticles

KW - lung epithelial cells

KW - nanoparticle shape

KW - nanotoxicology

KW - reactive oxygen species

UR - https://www.scopus.com/pages/publications/84941886516

U2 - 10.2217/nnm.15.103

DO - 10.2217/nnm.15.103

M3 - Article

C2 - 26377045

AN - SCOPUS:84941886516

SN - 1743-5889

VL - 10

SP - 2643

EP - 2657

JO - Nanomedicine

JF - Nanomedicine

IS - 17

ER -

Investigating the role of shape on the biological impact of gold nanoparticles in vitro

Abstract

Keywords

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