Infrared spectroscopy is suitable for objective assessment of articular cartilage health

V. Virtanen; V. Tafintseva; R. Shaikh; E. Nippolainen; J. Haas; I. O. Afara; J. Töyräs; H. Kröger; J. Solheim; B. Zimmermann; A. Kohler; B. Mizaikoff; M. Finnilä; L. Rieppo; S. Saarakkala

doi:10.1016/j.ocarto.2022.100250

Infrared spectroscopy is suitable for objective assessment of articular cartilage health

V. Virtanen, V. Tafintseva, R. Shaikh, E. Nippolainen, J. Haas, I. O. Afara, J. Töyräs, H. Kröger, J. Solheim, B. Zimmermann, A. Kohler, B. Mizaikoff, M. Finnilä, L. Rieppo, S. Saarakkala

Radiation and Environmental Science Centre (RESC)

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To evaluate the feasibility of Fourier transform infrared attenuated total reflectance (FTIR-ATR) spectroscopy to detect cartilage degradation due to osteoarthritis and to validate the methodology with osteochondral human cartilage samples for future development towards clinical use. Design: Cylindrical (d = 4 mm) osteochondral samples (n = 349) were prepared from nine human cadavers and measured with FTIR-ATR spectroscopy. Afterwards, the samples were assessed with Osteoarthritis Research Society International (OARSI) osteoarthritis cartilage histopathology assessment system and divided into two groups: 1) healthy (OARSI 0–2) and 2) osteoarthritic (OARSI 2.5–6). The classification was done with partial least squares discriminant analysis model utilizing cross-model validation. Receiver operating characteristics curve analysis was performed and the area under curve (AUC) was calculated. Results: For all samples combined, classification accuracy was 73% with AUC of 0.79. Femoral samples had accuracy of 74% and AUC of 0.77, while tibial samples had accuracy of 66%, and AUC of 0.74. Patellar samples had accuracy of 84% and AUC of 0.91. Conclusions: The results indicate that FTIR-ATR spectroscopy can differentiate between healthy and osteoarthritic femoral, tibial and patellar human tissue. If combined with a fiber optic probe, FTIR-ATR spectroscopy could provide additional objective intraoperative information during arthroscopic surgeries, which could improve clinical outcomes.

Original language	English
Article number	100250
Journal	Osteoarthritis and Cartilage Open
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/j.ocarto.2022.100250
Publication status	Published - Jun 2022

Keywords

Arthroscopy
Cartilage
Fourier transform infrared spectroscopy
Osteoarthritis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ocarto.2022.100250Licence: CC BY-NC-ND

1-s2.0-S2665913122000188-mainFinal published version, 845 KBLicence: CC BY-NC-ND

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Virtanen, V., Tafintseva, V., Shaikh, R., Nippolainen, E., Haas, J., Afara, I. O., Töyräs, J., Kröger, H., Solheim, J., Zimmermann, B., Kohler, A., Mizaikoff, B., Finnilä, M., Rieppo, L., & Saarakkala, S. (2022). Infrared spectroscopy is suitable for objective assessment of articular cartilage health. Osteoarthritis and Cartilage Open, 4(2), Article 100250. https://doi.org/10.1016/j.ocarto.2022.100250

@article{11381bce95fd4cf0931be2e67f27aadf,

title = "Infrared spectroscopy is suitable for objective assessment of articular cartilage health",

abstract = "Objective: To evaluate the feasibility of Fourier transform infrared attenuated total reflectance (FTIR-ATR) spectroscopy to detect cartilage degradation due to osteoarthritis and to validate the methodology with osteochondral human cartilage samples for future development towards clinical use. Design: Cylindrical (d = 4 mm) osteochondral samples (n = 349) were prepared from nine human cadavers and measured with FTIR-ATR spectroscopy. Afterwards, the samples were assessed with Osteoarthritis Research Society International (OARSI) osteoarthritis cartilage histopathology assessment system and divided into two groups: 1) healthy (OARSI 0–2) and 2) osteoarthritic (OARSI 2.5–6). The classification was done with partial least squares discriminant analysis model utilizing cross-model validation. Receiver operating characteristics curve analysis was performed and the area under curve (AUC) was calculated. Results: For all samples combined, classification accuracy was 73\% with AUC of 0.79. Femoral samples had accuracy of 74\% and AUC of 0.77, while tibial samples had accuracy of 66\%, and AUC of 0.74. Patellar samples had accuracy of 84\% and AUC of 0.91. Conclusions: The results indicate that FTIR-ATR spectroscopy can differentiate between healthy and osteoarthritic femoral, tibial and patellar human tissue. If combined with a fiber optic probe, FTIR-ATR spectroscopy could provide additional objective intraoperative information during arthroscopic surgeries, which could improve clinical outcomes.",

keywords = "Arthroscopy, Cartilage, Fourier transform infrared spectroscopy, Osteoarthritis",

author = "V. Virtanen and V. Tafintseva and R. Shaikh and E. Nippolainen and J. Haas and Afara, \{I. O.\} and J. T{\"o}yr{\"a}s and H. Kr{\"o}ger and J. Solheim and B. Zimmermann and A. Kohler and B. Mizaikoff and M. Finnil{\"a} and L. Rieppo and S. Saarakkala",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = jun,

doi = "10.1016/j.ocarto.2022.100250",

language = "English",

volume = "4",

journal = "Osteoarthritis and Cartilage Open",

issn = "2665-9131",

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number = "2",

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Virtanen, V, Tafintseva, V, Shaikh, R, Nippolainen, E, Haas, J, Afara, IO, Töyräs, J, Kröger, H, Solheim, J, Zimmermann, B, Kohler, A, Mizaikoff, B, Finnilä, M, Rieppo, L & Saarakkala, S 2022, 'Infrared spectroscopy is suitable for objective assessment of articular cartilage health', Osteoarthritis and Cartilage Open, vol. 4, no. 2, 100250. https://doi.org/10.1016/j.ocarto.2022.100250

TY - JOUR

T1 - Infrared spectroscopy is suitable for objective assessment of articular cartilage health

AU - Virtanen, V.

AU - Tafintseva, V.

AU - Shaikh, R.

AU - Nippolainen, E.

AU - Haas, J.

AU - Afara, I. O.

AU - Töyräs, J.

AU - Kröger, H.

AU - Solheim, J.

AU - Zimmermann, B.

AU - Kohler, A.

AU - Mizaikoff, B.

AU - Finnilä, M.

AU - Rieppo, L.

AU - Saarakkala, S.

PY - 2022/6

Y1 - 2022/6

N2 - Objective: To evaluate the feasibility of Fourier transform infrared attenuated total reflectance (FTIR-ATR) spectroscopy to detect cartilage degradation due to osteoarthritis and to validate the methodology with osteochondral human cartilage samples for future development towards clinical use. Design: Cylindrical (d = 4 mm) osteochondral samples (n = 349) were prepared from nine human cadavers and measured with FTIR-ATR spectroscopy. Afterwards, the samples were assessed with Osteoarthritis Research Society International (OARSI) osteoarthritis cartilage histopathology assessment system and divided into two groups: 1) healthy (OARSI 0–2) and 2) osteoarthritic (OARSI 2.5–6). The classification was done with partial least squares discriminant analysis model utilizing cross-model validation. Receiver operating characteristics curve analysis was performed and the area under curve (AUC) was calculated. Results: For all samples combined, classification accuracy was 73% with AUC of 0.79. Femoral samples had accuracy of 74% and AUC of 0.77, while tibial samples had accuracy of 66%, and AUC of 0.74. Patellar samples had accuracy of 84% and AUC of 0.91. Conclusions: The results indicate that FTIR-ATR spectroscopy can differentiate between healthy and osteoarthritic femoral, tibial and patellar human tissue. If combined with a fiber optic probe, FTIR-ATR spectroscopy could provide additional objective intraoperative information during arthroscopic surgeries, which could improve clinical outcomes.

AB - Objective: To evaluate the feasibility of Fourier transform infrared attenuated total reflectance (FTIR-ATR) spectroscopy to detect cartilage degradation due to osteoarthritis and to validate the methodology with osteochondral human cartilage samples for future development towards clinical use. Design: Cylindrical (d = 4 mm) osteochondral samples (n = 349) were prepared from nine human cadavers and measured with FTIR-ATR spectroscopy. Afterwards, the samples were assessed with Osteoarthritis Research Society International (OARSI) osteoarthritis cartilage histopathology assessment system and divided into two groups: 1) healthy (OARSI 0–2) and 2) osteoarthritic (OARSI 2.5–6). The classification was done with partial least squares discriminant analysis model utilizing cross-model validation. Receiver operating characteristics curve analysis was performed and the area under curve (AUC) was calculated. Results: For all samples combined, classification accuracy was 73% with AUC of 0.79. Femoral samples had accuracy of 74% and AUC of 0.77, while tibial samples had accuracy of 66%, and AUC of 0.74. Patellar samples had accuracy of 84% and AUC of 0.91. Conclusions: The results indicate that FTIR-ATR spectroscopy can differentiate between healthy and osteoarthritic femoral, tibial and patellar human tissue. If combined with a fiber optic probe, FTIR-ATR spectroscopy could provide additional objective intraoperative information during arthroscopic surgeries, which could improve clinical outcomes.

KW - Arthroscopy

KW - Cartilage

KW - Fourier transform infrared spectroscopy

KW - Osteoarthritis

UR - https://www.scopus.com/pages/publications/85149211454

U2 - 10.1016/j.ocarto.2022.100250

DO - 10.1016/j.ocarto.2022.100250

M3 - Article

AN - SCOPUS:85149211454

SN - 2665-9131

VL - 4

JO - Osteoarthritis and Cartilage Open

JF - Osteoarthritis and Cartilage Open

IS - 2

M1 - 100250

ER -

Infrared spectroscopy is suitable for objective assessment of articular cartilage health

Abstract

Keywords

UN SDGs

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