TY - JOUR
T1 - Infrared Based Saliva Screening Test for COVID-19
AU - Wood, Bayden R.
AU - Kochan, Kamila
AU - Bedolla, Diana E.
AU - Salazar-Quiroz, Natalia
AU - Grimley, Samantha L.
AU - Perez-Guaita, David
AU - Baker, Matthew J.
AU - Vongsvivut, Jitraporn
AU - Tobin, Mark J.
AU - Bambery, Keith R.
AU - Christensen, Dale
AU - Pasricha, Shivani
AU - Eden, Anthony K.
AU - Mclean, Aaron
AU - Roy, Supti
AU - Roberts, Jason A.
AU - Druce, Julian
AU - Williamson, Deborah A.
AU - McAuley, Julie
AU - Catton, Mike
AU - Purcell, Damian F.J.
AU - Godfrey, Dale I.
AU - Heraud, Philip
N1 - Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/7/26
Y1 - 2021/7/26
N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.
AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.
KW - COVID-19 diagnostic
KW - Fourier transform infrared (FTIR) spectroscopy
KW - Raman spectroscopy
KW - SARS-CoV-2
KW - saliva
UR - http://www.scopus.com/inward/record.url?scp=85108856948&partnerID=8YFLogxK
U2 - 10.1002/anie.202104453
DO - 10.1002/anie.202104453
M3 - Article
SN - 1433-7851
VL - 60
SP - 17102
EP - 17107
JO - Angewandte Chemie International Edition in English
JF - Angewandte Chemie International Edition in English
IS - 31
ER -