TY - JOUR
T1 - Influence of molecular weight fractionation on the antimicrobial and anticancer properties of a fucoidan rich-extract from the macroalgae Fucus vesiculosus
AU - Cabral, Eduarda M.
AU - Mondala, Julie Rose Mae
AU - Oliveira, Márcia
AU - Przyborska, Joanna
AU - Fitzpatrick, Stephen
AU - Rai, Dilip K.
AU - Sivagnanam, Saravana Periaswamy
AU - Garcia-Vaquero, Marco
AU - O'Shea, Denis
AU - Devereux, Michael
AU - Tiwari, Brijesh K.
AU - Curtin, James
N1 - Publisher Copyright:
© 2021 The Authors
PY - 2021/9/1
Y1 - 2021/9/1
N2 - The objective of this study was to investigate the antimicrobial and anticancer properties of a fucoidan extract and subsequent fractions isolated from the macroalgae Fucus vesiculosus. The fractions obtained (>300 kDa, <300 kDa, <100 kDa, <50 kDa and <10 kDa) could inhibit the growth of B. subtilis, E. coli, L. innocua and P. fluorescens when assayed at concentrations between 12,500 and 25,000 ppm. The bacterial growth was monitored by optical density (OD) measurements (600 nm, 24 h) at 30 °C or 37 °C, depending upon on the strain used. The extracted fractions were also tested for cytotoxicity against brain glioblastoma cancer cells using the Alamar Blue assay for 24 h, 48 h and 6 days. The >300 kDa fraction presented the lowest IC50 values (0.052% - 24 h; 0.032% - 6 days). The potential bioactivity of fucoidan as an antimicrobial and anticancer agent was demonstrated in this study. Hence, the related mechanisms of action should be explored in a near future.
AB - The objective of this study was to investigate the antimicrobial and anticancer properties of a fucoidan extract and subsequent fractions isolated from the macroalgae Fucus vesiculosus. The fractions obtained (>300 kDa, <300 kDa, <100 kDa, <50 kDa and <10 kDa) could inhibit the growth of B. subtilis, E. coli, L. innocua and P. fluorescens when assayed at concentrations between 12,500 and 25,000 ppm. The bacterial growth was monitored by optical density (OD) measurements (600 nm, 24 h) at 30 °C or 37 °C, depending upon on the strain used. The extracted fractions were also tested for cytotoxicity against brain glioblastoma cancer cells using the Alamar Blue assay for 24 h, 48 h and 6 days. The >300 kDa fraction presented the lowest IC50 values (0.052% - 24 h; 0.032% - 6 days). The potential bioactivity of fucoidan as an antimicrobial and anticancer agent was demonstrated in this study. Hence, the related mechanisms of action should be explored in a near future.
KW - Anticancer properties
KW - Antimicrobial properties
KW - Brown macroalgae
KW - Fucoidan
KW - Laminarin
KW - Weight fractionation
UR - http://www.scopus.com/inward/record.url?scp=85111010077&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2021.06.182
DO - 10.1016/j.ijbiomac.2021.06.182
M3 - Article
C2 - 34216667
AN - SCOPUS:85111010077
SN - 0141-8130
VL - 186
SP - 994
EP - 1002
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -