TY - JOUR
T1 - In vitro localisation and degradation of few-layer MoS2 submicrometric plates in human macrophage-like cells
T2 - A label free Raman micro-spectroscopic study
AU - Moore, Caroline
AU - Harvey, Andrew
AU - Coleman, Jonathan N.
AU - Byrne, Hugh J.
AU - McIntyre, Jennifer
N1 - Publisher Copyright:
© 2020 IOP Publishing Ltd.
PY - 2020
Y1 - 2020
N2 - Monitoring the uptake, micro-environment and fate of micro or nano scaled particulate materials in cells is of paramount importance for the emerging fields of toxicology and medicine. Such particulate materials are known to interfere with colorimetric assays and many such assays record only a single end-point. Therefore, there is a need for a label-free, cost effective technique with little or no inference from the particulate materials. Raman micro-spectroscopy was used to simultaneously interrogate the integrity of few-layer MoS2 submicrometric plates in human macrophage-like cells, in vitro, as well as the biochemical characteristics of the local micro-environment in which they are encompassed. Firstly, the degradation profile of MoS2 plates induced by hydrogen peroxidase was established using UV-vis absorption and Raman micro-spectroscopy. Raman micro-spectroscopic maps interrogated all aspects of the cell, including the nucleus, cytoplasm and perinuclear region, and the location/distribution of MoS2 was monitored as a function of time (4, 24 and 72 h). Whereas only pristine MoS2 was detectable after 4 and 72 periods, degradation in vitro was confirmed following a 24 h incubation. Analysis of the MoS2 micro-environments revealed the presence of both phosphatidyl lipidic vesicles and enzymatic regions containing lysozyme, the former being most associated with the MoS2 degradation. There was an increase and saturation of cytosolic neutral lipids detected following a 24 h incubation with MoS2, which reduces following a prolonged incubation of 72 h.
AB - Monitoring the uptake, micro-environment and fate of micro or nano scaled particulate materials in cells is of paramount importance for the emerging fields of toxicology and medicine. Such particulate materials are known to interfere with colorimetric assays and many such assays record only a single end-point. Therefore, there is a need for a label-free, cost effective technique with little or no inference from the particulate materials. Raman micro-spectroscopy was used to simultaneously interrogate the integrity of few-layer MoS2 submicrometric plates in human macrophage-like cells, in vitro, as well as the biochemical characteristics of the local micro-environment in which they are encompassed. Firstly, the degradation profile of MoS2 plates induced by hydrogen peroxidase was established using UV-vis absorption and Raman micro-spectroscopy. Raman micro-spectroscopic maps interrogated all aspects of the cell, including the nucleus, cytoplasm and perinuclear region, and the location/distribution of MoS2 was monitored as a function of time (4, 24 and 72 h). Whereas only pristine MoS2 was detectable after 4 and 72 periods, degradation in vitro was confirmed following a 24 h incubation. Analysis of the MoS2 micro-environments revealed the presence of both phosphatidyl lipidic vesicles and enzymatic regions containing lysozyme, the former being most associated with the MoS2 degradation. There was an increase and saturation of cytosolic neutral lipids detected following a 24 h incubation with MoS2, which reduces following a prolonged incubation of 72 h.
KW - MoSsubmicrometric plates
KW - Raman micro-spectroscopy
KW - cellular uptake8sub-cellular location
KW - material integrity
KW - submicrometric plates degradation
UR - http://www.scopus.com/inward/record.url?scp=85082513974&partnerID=8YFLogxK
U2 - 10.1088/2053-1583/ab5d98
DO - 10.1088/2053-1583/ab5d98
M3 - Article
AN - SCOPUS:85082513974
SN - 2053-1583
VL - 7
JO - 2D Materials
JF - 2D Materials
IS - 2
M1 - 025003
ER -