In vitro cytotoxic potential and mechanism of action of selected coumarins, using human renal cell lines

Gregory J. Finn; Emma Kenealy; Bernadette S. Creaven; Denise A. Egan

doi:10.1016/S0304-3835(02)00102-7

In vitro cytotoxic potential and mechanism of action of selected coumarins, using human renal cell lines

Gregory J. Finn, Emma Kenealy, Bernadette S. Creaven, Denise A. Egan

Research output: Contribution to journal › Article › peer-review

Abstract

This study determined the selective cytotoxicity of eight coumarin compounds to human renal carcinoma cells, relative to non-carcinoma proximal tubular cells. Selectivity cytotoxicity was observed following exposure to 6-nitro-7-hydroxycoumarin (6-NO₂-7-OHC) and 7,8-dihydroxycoumarin (7,8-OHC). 6-NO₂-7-OHC induced cytotoxicity was irreversible in both cell lines, unlike 7,8-OHC, which was reversible in the carcinoma cells only. Mobility shift and BrdU incorporation assays showed that both compounds did not intercalate DNA but had a concentration-dependent inhibitory effect on its synthesis. All coumarins studied were found to be non-mutagenic using the standard Ames test. These results would suggest that 6-NO₂-7-OHC and 7,8-OHC might have a therapeutic role to play in the treatment of renal cell carcinoma.

Original language	English
Pages (from-to)	61-68
Number of pages	8
Journal	Cancer Letters
Volume	183
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(02)00102-7
Publication status	Published - 8 Sep 2002
Externally published	Yes

Keywords

Daphnetin
Esculetin
Nitrocoumarins
Renal cell carcinoma

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10.1016/S0304-3835(02)00102-7

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title = "In vitro cytotoxic potential and mechanism of action of selected coumarins, using human renal cell lines",

abstract = "This study determined the selective cytotoxicity of eight coumarin compounds to human renal carcinoma cells, relative to non-carcinoma proximal tubular cells. Selectivity cytotoxicity was observed following exposure to 6-nitro-7-hydroxycoumarin (6-NO2-7-OHC) and 7,8-dihydroxycoumarin (7,8-OHC). 6-NO2-7-OHC induced cytotoxicity was irreversible in both cell lines, unlike 7,8-OHC, which was reversible in the carcinoma cells only. Mobility shift and BrdU incorporation assays showed that both compounds did not intercalate DNA but had a concentration-dependent inhibitory effect on its synthesis. All coumarins studied were found to be non-mutagenic using the standard Ames test. These results would suggest that 6-NO2-7-OHC and 7,8-OHC might have a therapeutic role to play in the treatment of renal cell carcinoma.",

keywords = "Daphnetin, Esculetin, Nitrocoumarins, Renal cell carcinoma",

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T1 - In vitro cytotoxic potential and mechanism of action of selected coumarins, using human renal cell lines

AU - Finn, Gregory J.

AU - Kenealy, Emma

AU - Creaven, Bernadette S.

AU - Egan, Denise A.

PY - 2002/9/8

Y1 - 2002/9/8

N2 - This study determined the selective cytotoxicity of eight coumarin compounds to human renal carcinoma cells, relative to non-carcinoma proximal tubular cells. Selectivity cytotoxicity was observed following exposure to 6-nitro-7-hydroxycoumarin (6-NO2-7-OHC) and 7,8-dihydroxycoumarin (7,8-OHC). 6-NO2-7-OHC induced cytotoxicity was irreversible in both cell lines, unlike 7,8-OHC, which was reversible in the carcinoma cells only. Mobility shift and BrdU incorporation assays showed that both compounds did not intercalate DNA but had a concentration-dependent inhibitory effect on its synthesis. All coumarins studied were found to be non-mutagenic using the standard Ames test. These results would suggest that 6-NO2-7-OHC and 7,8-OHC might have a therapeutic role to play in the treatment of renal cell carcinoma.

AB - This study determined the selective cytotoxicity of eight coumarin compounds to human renal carcinoma cells, relative to non-carcinoma proximal tubular cells. Selectivity cytotoxicity was observed following exposure to 6-nitro-7-hydroxycoumarin (6-NO2-7-OHC) and 7,8-dihydroxycoumarin (7,8-OHC). 6-NO2-7-OHC induced cytotoxicity was irreversible in both cell lines, unlike 7,8-OHC, which was reversible in the carcinoma cells only. Mobility shift and BrdU incorporation assays showed that both compounds did not intercalate DNA but had a concentration-dependent inhibitory effect on its synthesis. All coumarins studied were found to be non-mutagenic using the standard Ames test. These results would suggest that 6-NO2-7-OHC and 7,8-OHC might have a therapeutic role to play in the treatment of renal cell carcinoma.

KW - Daphnetin

KW - Esculetin

KW - Nitrocoumarins

KW - Renal cell carcinoma

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AN - SCOPUS:0037044111

SN - 0304-3835

VL - 183

SP - 61

EP - 68

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

In vitro cytotoxic potential and mechanism of action of selected coumarins, using human renal cell lines

Abstract

Keywords

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