Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice

Anna A. Shvedova; Alexandr A. Kapralov; Wei Hong Feng; Elena R. Kisin; Ashley R. Murray; Robert R. Mercer; Claudette M. St. Croix; Megan A. Lang; Simon C. Watkins; Nagarjun V. Konduru; Brett L. Allen; Jennifer Conroy; Gregg P. Kotchey; Bashir M. Mohamed; Aidan D. Meade; Yuri Volkov; Alexander Star; Bengt Fadeel; Valerian E. Kagan

doi:10.1371/journal.pone.0030923

Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice

Anna A. Shvedova, Alexandr A. Kapralov, Wei Hong Feng, Elena R. Kisin, Ashley R. Murray, Robert R. Mercer, Claudette M. St. Croix, Megan A. Lang, Simon C. Watkins, Nagarjun V. Konduru, Brett L. Allen, Jennifer Conroy, Gregg P. Kotchey, Bashir M. Mohamed, Aidan D. Meade, Yuri Volkov, Alexander Star, Bengt Fadeel, Valerian E. Kagan

Research output: Contribution to journal › Article › peer-review

Abstract

Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations.

Original language	English
Article number	e30923
Journal	PLoS ONE
Volume	7
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0030923
Publication status	Published - 30 Mar 2012

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Shvedova, A. A., Kapralov, A. A., Feng, W. H., Kisin, E. R., Murray, A. R., Mercer, R. R., St. Croix, C. M., Lang, M. A., Watkins, S. C., Konduru, N. V., Allen, B. L., Conroy, J., Kotchey, G. P., Mohamed, B. M., Meade, A. D., Volkov, Y., Star, A., Fadeel, B., & Kagan, V. E. (2012). Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice. PLoS ONE, 7(3), Article e30923. https://doi.org/10.1371/journal.pone.0030923

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title = "Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice",

abstract = "Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations.",

author = "Shvedova, {Anna A.} and Kapralov, {Alexandr A.} and Feng, {Wei Hong} and Kisin, {Elena R.} and Murray, {Ashley R.} and Mercer, {Robert R.} and {St. Croix}, {Claudette M.} and Lang, {Megan A.} and Watkins, {Simon C.} and Konduru, {Nagarjun V.} and Allen, {Brett L.} and Jennifer Conroy and Kotchey, {Gregg P.} and Mohamed, {Bashir M.} and Meade, {Aidan D.} and Yuri Volkov and Alexander Star and Bengt Fadeel and Kagan, {Valerian E.}",

year = "2012",

month = mar,

day = "30",

doi = "10.1371/journal.pone.0030923",

language = "English",

volume = "7",

journal = "PLoS ONE",

issn = "1932-6203",

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Shvedova, AA, Kapralov, AA, Feng, WH, Kisin, ER, Murray, AR, Mercer, RR, St. Croix, CM, Lang, MA, Watkins, SC, Konduru, NV, Allen, BL, Conroy, J, Kotchey, GP, Mohamed, BM, Meade, AD, Volkov, Y, Star, A, Fadeel, B & Kagan, VE 2012, 'Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice', PLoS ONE, vol. 7, no. 3, e30923. https://doi.org/10.1371/journal.pone.0030923

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AU - Shvedova, Anna A.

AU - Kapralov, Alexandr A.

AU - Feng, Wei Hong

AU - Kisin, Elena R.

AU - Murray, Ashley R.

AU - Mercer, Robert R.

AU - St. Croix, Claudette M.

AU - Lang, Megan A.

AU - Watkins, Simon C.

AU - Konduru, Nagarjun V.

AU - Allen, Brett L.

AU - Conroy, Jennifer

AU - Kotchey, Gregg P.

AU - Mohamed, Bashir M.

AU - Meade, Aidan D.

AU - Volkov, Yuri

AU - Star, Alexander

AU - Fadeel, Bengt

AU - Kagan, Valerian E.

PY - 2012/3/30

Y1 - 2012/3/30

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AB - Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations.

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Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice

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