Immobilization of conidiobolus coronatus alkaline protease on waste fungal biomass

Shiv Shankar, Ryali Seeta Laxman

Research output: Contribution to journalArticlepeer-review

Abstract

Alkaline protease from Conidiobolus coronatus which is optimally active at pH 10 and 40°C finds application in leather and detergent industries as well as for recovery of silver from waste photographic films. The protease was immobilized in Ca-alginate, polyacrylamide gel and alkali treated waste fungal biomass (ATWFB). ATWFB was found to be most suitable among the matrices tested. Glutaraldehyde marginally increased the binding to ATWFB. Binding of protease to ATWFB seems to be through adsorption as confirmed by FTIR spectra. Though temperature optima of free and immobilized proteases were identical, optimum pH of the immobilized enzyme shifted to 11 from 10. Temperature stability of the protease increased after immobilization. Immobilized protease could be reused 3 times with marginal loss in activity.

Original languageEnglish
Pages (from-to)1727-1732
Number of pages6
JournalEnvironmental Engineering and Management Journal
Volume10
Issue number11
DOIs
Publication statusPublished - Nov 2011
Externally publishedYes

Keywords

  • Alkaline protease
  • Conidiobolus coronatus
  • Fungal biomass
  • Immobilization

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