Green synthesis of silver nanoribbons from waste X-ray films using alkaline protease

Shiv Shankar; R. G.S.V. Prasad; P. R. Selvakannan; Lily Jaiswal; R. S. Laxman

doi:10.1166/mex.2015.1221

Green synthesis of silver nanoribbons from waste X-ray films using alkaline protease

Shiv Shankar, R. G.S.V. Prasad, P. R. Selvakannan, Lily Jaiswal, R. S. Laxman

Research output: Contribution to journal › Article › peer-review

Abstract

This work illustrates a facile approach to synthesize silver nanoribbons from waste X-ray films using alkaline protease from Beauveria sp. MTCC 5184 (BAP). The green synthesized nanoribbons have been characterized by UV-Vis spectroscopy, TEM, and FTIR. The UV-visible spectra gave maximum absorbance at 400 nm. TEM micrograph showed well-dispersed silver nanoribbons with an average length of 200–400 nm. FTIR result demonstrated the respective bands of the silver nanoribbons and proteins. XRD data revealed crystalline nature of silver nanoribbons dominated with (111) facets. In vitro antibacterial activity in terms of minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) showed 8/16 μg/mL (MIC/MBC) against Staphylococcus aureus ATCC 29213 and 4/8 μg/mL (MIC/MBC) against Escherichia coli ATCC 25922. The green synthesized silver nanoribbons showed excellent biocompatibility up to 40 μg/mL concentrations on mouse fibroblast cell line (L929).

Original language	English
Pages (from-to)	165-170
Number of pages	6
Journal	Materials Express
Volume	5
Issue number	2
DOIs	https://doi.org/10.1166/mex.2015.1221
Publication status	Published - 2015
Externally published	Yes

Keywords

Alkaline protease
Characterization
Cytotoxicity
Silver nanoribbons
Waste X-ray film

Access to Document

10.1166/mex.2015.1221

Cite this

@article{1dc05ec09f4c429a9a00b89f4d43d575,

title = "Green synthesis of silver nanoribbons from waste X-ray films using alkaline protease",

abstract = "This work illustrates a facile approach to synthesize silver nanoribbons from waste X-ray films using alkaline protease from Beauveria sp. MTCC 5184 (BAP). The green synthesized nanoribbons have been characterized by UV-Vis spectroscopy, TEM, and FTIR. The UV-visible spectra gave maximum absorbance at 400 nm. TEM micrograph showed well-dispersed silver nanoribbons with an average length of 200–400 nm. FTIR result demonstrated the respective bands of the silver nanoribbons and proteins. XRD data revealed crystalline nature of silver nanoribbons dominated with (111) facets. In vitro antibacterial activity in terms of minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) showed 8/16 μg/mL (MIC/MBC) against Staphylococcus aureus ATCC 29213 and 4/8 μg/mL (MIC/MBC) against Escherichia coli ATCC 25922. The green synthesized silver nanoribbons showed excellent biocompatibility up to 40 μg/mL concentrations on mouse fibroblast cell line (L929).",

keywords = "Alkaline protease, Characterization, Cytotoxicity, Silver nanoribbons, Waste X-ray film",

author = "Shiv Shankar and Prasad, {R. G.S.V.} and Selvakannan, {P. R.} and Lily Jaiswal and Laxman, {R. S.}",

year = "2015",

doi = "10.1166/mex.2015.1221",

language = "English",

volume = "5",

pages = "165--170",

journal = "Materials Express",

issn = "2158-5849",

publisher = "American Scientific Publishers",

number = "2",

}

TY - JOUR

T1 - Green synthesis of silver nanoribbons from waste X-ray films using alkaline protease

AU - Shankar, Shiv

AU - Prasad, R. G.S.V.

AU - Selvakannan, P. R.

AU - Jaiswal, Lily

AU - Laxman, R. S.

PY - 2015

Y1 - 2015

N2 - This work illustrates a facile approach to synthesize silver nanoribbons from waste X-ray films using alkaline protease from Beauveria sp. MTCC 5184 (BAP). The green synthesized nanoribbons have been characterized by UV-Vis spectroscopy, TEM, and FTIR. The UV-visible spectra gave maximum absorbance at 400 nm. TEM micrograph showed well-dispersed silver nanoribbons with an average length of 200–400 nm. FTIR result demonstrated the respective bands of the silver nanoribbons and proteins. XRD data revealed crystalline nature of silver nanoribbons dominated with (111) facets. In vitro antibacterial activity in terms of minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) showed 8/16 μg/mL (MIC/MBC) against Staphylococcus aureus ATCC 29213 and 4/8 μg/mL (MIC/MBC) against Escherichia coli ATCC 25922. The green synthesized silver nanoribbons showed excellent biocompatibility up to 40 μg/mL concentrations on mouse fibroblast cell line (L929).

AB - This work illustrates a facile approach to synthesize silver nanoribbons from waste X-ray films using alkaline protease from Beauveria sp. MTCC 5184 (BAP). The green synthesized nanoribbons have been characterized by UV-Vis spectroscopy, TEM, and FTIR. The UV-visible spectra gave maximum absorbance at 400 nm. TEM micrograph showed well-dispersed silver nanoribbons with an average length of 200–400 nm. FTIR result demonstrated the respective bands of the silver nanoribbons and proteins. XRD data revealed crystalline nature of silver nanoribbons dominated with (111) facets. In vitro antibacterial activity in terms of minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) showed 8/16 μg/mL (MIC/MBC) against Staphylococcus aureus ATCC 29213 and 4/8 μg/mL (MIC/MBC) against Escherichia coli ATCC 25922. The green synthesized silver nanoribbons showed excellent biocompatibility up to 40 μg/mL concentrations on mouse fibroblast cell line (L929).

KW - Alkaline protease

KW - Characterization

KW - Cytotoxicity

KW - Silver nanoribbons

KW - Waste X-ray film

UR - http://www.scopus.com/inward/record.url?scp=84924135221&partnerID=8YFLogxK

U2 - 10.1166/mex.2015.1221

DO - 10.1166/mex.2015.1221

M3 - Article

AN - SCOPUS:84924135221

SN - 2158-5849

VL - 5

SP - 165

EP - 170

JO - Materials Express

JF - Materials Express

IS - 2

ER -

Green synthesis of silver nanoribbons from waste X-ray films using alkaline protease

Abstract

Keywords

Access to Document

Fingerprint

Cite this