TY - JOUR
T1 - Graphene nanoflake uptake mediated by scavenger receptors
AU - Alnasser, Fatima
AU - Castagnola, Valentina
AU - Boselli, Luca
AU - Esquivel-Gaon, Margarita
AU - Efeoglu, Esen
AU - McIntyre, Jennifer
AU - Byrne, Hugh J.
AU - Dawson, Kenneth A.
N1 - Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/2/13
Y1 - 2019/2/13
N2 - The biological interactions of graphene have been extensively investigated over the last 10 years. However, very little is known about graphene interactions with the cell surface and how the graphene internalization process is driven and mediated by specific recognition sites at the interface with the cell. In this work, we propose a methodology to investigate direct molecular correlations between the biomolecular corona of graphene and specific cell receptors, showing that key protein recognition motifs, presented on the nanomaterial surface, can engage selectively with specific cell receptors. We consider the case of apolipoprotein A-I, found to be very abundant in the graphene protein corona, and observe that the uptake of graphene nanoflakes is somewhat increased in cells with greatly elevated expression of scavenger receptors B1, suggesting a possible mechanism of endogenous interaction. The uptake results, obtained by flow cytometry, have been confirmed using Raman microspectroscopic mapping, exploiting the strong Raman signature of graphene.
AB - The biological interactions of graphene have been extensively investigated over the last 10 years. However, very little is known about graphene interactions with the cell surface and how the graphene internalization process is driven and mediated by specific recognition sites at the interface with the cell. In this work, we propose a methodology to investigate direct molecular correlations between the biomolecular corona of graphene and specific cell receptors, showing that key protein recognition motifs, presented on the nanomaterial surface, can engage selectively with specific cell receptors. We consider the case of apolipoprotein A-I, found to be very abundant in the graphene protein corona, and observe that the uptake of graphene nanoflakes is somewhat increased in cells with greatly elevated expression of scavenger receptors B1, suggesting a possible mechanism of endogenous interaction. The uptake results, obtained by flow cytometry, have been confirmed using Raman microspectroscopic mapping, exploiting the strong Raman signature of graphene.
KW - Graphene
KW - Nanobiological interactions
KW - Protein corona
KW - Scavenger receptors
UR - http://www.scopus.com/inward/record.url?scp=85060291068&partnerID=8YFLogxK
U2 - 10.1021/acs.nanolett.8b04820
DO - 10.1021/acs.nanolett.8b04820
M3 - Article
C2 - 30628448
AN - SCOPUS:85060291068
SN - 1530-6984
VL - 19
SP - 1260
EP - 1268
JO - Nano Letters
JF - Nano Letters
IS - 2
ER -