Fluorescence quenching-based mechanism for determination of hypochlorite by coumarin-derived sensors

Karolina Starzak; Arkadiusz Matwijczuk; Bernadette Creaven; Alicja Matwijczuk; Sławomir Wybraniec; Dariusz Karcz

doi:10.3390/ijms20020281

Fluorescence quenching-based mechanism for determination of hypochlorite by coumarin-derived sensors

Karolina Starzak, Arkadiusz Matwijczuk, Bernadette Creaven, Alicja Matwijczuk, Sławomir Wybraniec, Dariusz Karcz

Technological University Dublin

Research output: Contribution to journal › Article › peer-review

Abstract

A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.

Original language	English
Article number	281
Journal	International Journal of Molecular Sciences
Volume	20
Issue number	2
DOIs	https://doi.org/10.3390/ijms20020281
Publication status	Published - 2 Jan 2019

Keywords

Coumarin
Fluorescence quenching
Hypochlorite sensing

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10.3390/ijms20020281

https://arrow.tudublin.ie/ittsciart/66Licence: CC BY-NC-SA

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title = "Fluorescence quenching-based mechanism for determination of hypochlorite by coumarin-derived sensors",

abstract = "A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.",

keywords = "Coumarin, Fluorescence quenching, Hypochlorite sensing",

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T1 - Fluorescence quenching-based mechanism for determination of hypochlorite by coumarin-derived sensors

AU - Starzak, Karolina

AU - Matwijczuk, Arkadiusz

AU - Creaven, Bernadette

AU - Matwijczuk, Alicja

AU - Wybraniec, Sławomir

AU - Karcz, Dariusz

PY - 2019/1/2

Y1 - 2019/1/2

N2 - A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.

AB - A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.

KW - Coumarin

KW - Fluorescence quenching

KW - Hypochlorite sensing

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JO - International Journal of Molecular Sciences

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Fluorescence quenching-based mechanism for determination of hypochlorite by coumarin-derived sensors

Abstract

Keywords

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