TY - JOUR
T1 - Fins-like tyrosine kinase 3 ligand recruits plasmacytoid dendritic cells to the brain
AU - Curtin, James F.
AU - King, Gwendalyn D.
AU - Barcia, Carlos
AU - Liu, Chunyan
AU - Hubert, François X.
AU - Guillonneau, Carole
AU - Josien, Régis
AU - Anegon, Ignacio
AU - Lowenstein, Pedro E.
AU - Castro, Maria G.
PY - 2006/3/15
Y1 - 2006/3/15
N2 - The lack of professional afferent APCs in naive brain parenchyma contributes to the systemic immune ignorance to Ags localized exclusively within the brain. Dendritic cells (DCs) appear within the brain as a consequence of inflammation, but no molecular mechanisms accounting for this influx have been described. In this study we demonstrate that Fms-like tyrosine kinase 3 ligand (Flt3) recruits plasmacytoid DCs (pDCs; >50-fold; p < 0.001) to the brain parenchyma. These pDCs expressed IFN-α, the hallmark cytokine produced by pDCs, indicating recruitment and activation in situ of bona fide pDCs within the brain parenchyma. Flt3L did not increase the numbers of conventional DCs, macrophages, or B, T, NK, NKT, or microglial cells within the brain. Our data demonstrate that FltL reconstitutes a crucial afferent component of the immune response, namely, professional APCs within the brain parenchyma, and this could counteract the intrinsic systemic immune ignorance to Ags localized exclusively within the brain.
AB - The lack of professional afferent APCs in naive brain parenchyma contributes to the systemic immune ignorance to Ags localized exclusively within the brain. Dendritic cells (DCs) appear within the brain as a consequence of inflammation, but no molecular mechanisms accounting for this influx have been described. In this study we demonstrate that Fms-like tyrosine kinase 3 ligand (Flt3) recruits plasmacytoid DCs (pDCs; >50-fold; p < 0.001) to the brain parenchyma. These pDCs expressed IFN-α, the hallmark cytokine produced by pDCs, indicating recruitment and activation in situ of bona fide pDCs within the brain parenchyma. Flt3L did not increase the numbers of conventional DCs, macrophages, or B, T, NK, NKT, or microglial cells within the brain. Our data demonstrate that FltL reconstitutes a crucial afferent component of the immune response, namely, professional APCs within the brain parenchyma, and this could counteract the intrinsic systemic immune ignorance to Ags localized exclusively within the brain.
UR - http://www.scopus.com/inward/record.url?scp=33644836881&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.176.6.3566
DO - 10.4049/jimmunol.176.6.3566
M3 - Article
C2 - 16517725
AN - SCOPUS:33644836881
SN - 0022-1767
VL - 176
SP - 3566
EP - 3577
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -