Evaluation of silver nanoparticle encapsulation in DPPC-based liposome by different methods for enhanced cytotoxicity

Azeez Yusuf, Alan Casey

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Here we carried out a comparative study on two cost and time effective methods of encapsulating silver nanoparticles (AgNP) in dipalmitoyl-phosphatidyl choline (DPPC)/cholesterol-based liposome to enhance nanoparticle cytotoxicity, and evaluated the effect of this on a blood cell line (THP1 monocytes) often involved in uptake of nanoparticles during human exposure. DLS and Zeta potential analyses over a 6-months period showed the extruded Lipo-AgNP (Ex-Lipo-AgNP) exhibited more stable characteristics when compared with the probe-sonicated Lipo-AgNP (PB-Lipo-AgNP). SEM microscopy indicated agglomeration of the PB-Lipo-AgNP which was not observed in Ex-Lipo-AgNP. Ex-Lipo-AgNP also exhibited higher temperature-dependent stability with 35.3% reduction in size from 20 °C to 37 °C while PB-Lipo-AgNP was less stable exhibiting 55% size reduction over same temperature range and 6 h period. Load release study over 24 h showed controlled release from Ex-Lipo-AgNP while the PB-Lipo-AgNP exhibited burst release at pH 4 and 6.5. Interestingly, it was found that Ex-Lipo-AgNP induced significantly higher cytotoxicity on THP1 cell line after 24 h exposure compared with control unexposed cells, uncoated AgNP and PB-Lipo-AgNP exposed cells at the same concentration. Thus, we report here that liposomal encapsulation of AgNP by extrusion produces a stable nanocapsule with enhanced cytotoxicity, thus preventing overreliance on high AgNP concentration to achieve desired toxicity for in vitro and possible in vivo applications.

Original languageEnglish
Pages (from-to)860-871
Number of pages12
JournalInternational Journal of Polymeric Materials and Polymeric Biomaterials
Volume69
Issue number13
DOIs
Publication statusPublished - 1 Sep 2020

Keywords

  • Silver nanoparticle (AgNP)
  • encapsulation
  • extrusion
  • liposome

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