Effects of Single-Walled Carbon Nanotube on Polymerase Chain Reaction

Daxiang Cui; Furong Tian; Yong Kong; Cengiz S. Ozkan; Igor Titushikin; Huajian Gao

doi:10.1557/proc-773-n2.3

Effects of Single-Walled Carbon Nanotube on Polymerase Chain Reaction

Daxiang Cui, Furong Tian, Yong Kong, Cengiz S. Ozkan, Igor Titushikin, Huajian Gao

Research output: Contribution to journal › Conference article › peer-review

Abstract

Polymerase Chain Reaction (PCR) is a complicated gene amplification process using Mg²⁺ as an assistant factor of Taq enzyme. Influence of single-walled carbon nanotube on that reaction was investigated with scanning electron microscope (SEM), transmission electron microscope (TEM) and quantitative PCR product analysis. Results showed that adding single-walled carbon nanotubes in the reaction liquid with a concentration of less than 3 μg/μl increase the amounts of PCR products. But more carbon nanotubes cause a great reduction of the PCR products. Similar effects were observed in the reaction without Mg²⁺. Possible reason could be the aggregation of reaction components caused by small amount of additive carbon nanotubes, which increases reaction probability. Our observations suggest that small amount of carbon nanotube could be used as an assistant factor to improve the PCR reaction.

Original language	English
Pages (from-to)	89-93
Number of pages	5
Journal	Materials Research Society Symposium - Proceedings
Volume	773
DOIs	https://doi.org/10.1557/proc-773-n2.3
Publication status	Published - 2003
Externally published	Yes
Event	Biomicroelectromechanical Systems (BioMEMS) - San Francisco, CA, United States Duration: 22 Apr 2003 → 25 Apr 2003

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title = "Effects of Single-Walled Carbon Nanotube on Polymerase Chain Reaction",

abstract = "Polymerase Chain Reaction (PCR) is a complicated gene amplification process using Mg2+ as an assistant factor of Taq enzyme. Influence of single-walled carbon nanotube on that reaction was investigated with scanning electron microscope (SEM), transmission electron microscope (TEM) and quantitative PCR product analysis. Results showed that adding single-walled carbon nanotubes in the reaction liquid with a concentration of less than 3 μg/μl increase the amounts of PCR products. But more carbon nanotubes cause a great reduction of the PCR products. Similar effects were observed in the reaction without Mg2+. Possible reason could be the aggregation of reaction components caused by small amount of additive carbon nanotubes, which increases reaction probability. Our observations suggest that small amount of carbon nanotube could be used as an assistant factor to improve the PCR reaction.",

author = "Daxiang Cui and Furong Tian and Yong Kong and Ozkan, {Cengiz S.} and Igor Titushikin and Huajian Gao",

year = "2003",

doi = "10.1557/proc-773-n2.3",

language = "English",

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pages = "89--93",

journal = "Materials Research Society Symposium - Proceedings",

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note = "Biomicroelectromechanical Systems (BioMEMS) ; Conference date: 22-04-2003 Through 25-04-2003",

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TY - JOUR

T1 - Effects of Single-Walled Carbon Nanotube on Polymerase Chain Reaction

AU - Cui, Daxiang

AU - Tian, Furong

AU - Kong, Yong

AU - Ozkan, Cengiz S.

AU - Titushikin, Igor

AU - Gao, Huajian

PY - 2003

Y1 - 2003

N2 - Polymerase Chain Reaction (PCR) is a complicated gene amplification process using Mg2+ as an assistant factor of Taq enzyme. Influence of single-walled carbon nanotube on that reaction was investigated with scanning electron microscope (SEM), transmission electron microscope (TEM) and quantitative PCR product analysis. Results showed that adding single-walled carbon nanotubes in the reaction liquid with a concentration of less than 3 μg/μl increase the amounts of PCR products. But more carbon nanotubes cause a great reduction of the PCR products. Similar effects were observed in the reaction without Mg2+. Possible reason could be the aggregation of reaction components caused by small amount of additive carbon nanotubes, which increases reaction probability. Our observations suggest that small amount of carbon nanotube could be used as an assistant factor to improve the PCR reaction.

AB - Polymerase Chain Reaction (PCR) is a complicated gene amplification process using Mg2+ as an assistant factor of Taq enzyme. Influence of single-walled carbon nanotube on that reaction was investigated with scanning electron microscope (SEM), transmission electron microscope (TEM) and quantitative PCR product analysis. Results showed that adding single-walled carbon nanotubes in the reaction liquid with a concentration of less than 3 μg/μl increase the amounts of PCR products. But more carbon nanotubes cause a great reduction of the PCR products. Similar effects were observed in the reaction without Mg2+. Possible reason could be the aggregation of reaction components caused by small amount of additive carbon nanotubes, which increases reaction probability. Our observations suggest that small amount of carbon nanotube could be used as an assistant factor to improve the PCR reaction.

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DO - 10.1557/proc-773-n2.3

M3 - Conference article

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SN - 0272-9172

VL - 773

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JO - Materials Research Society Symposium - Proceedings

JF - Materials Research Society Symposium - Proceedings

T2 - Biomicroelectromechanical Systems (BioMEMS)

Y2 - 22 April 2003 through 25 April 2003

ER -

Effects of Single-Walled Carbon Nanotube on Polymerase Chain Reaction

Abstract

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