Effects of four commercially available factor Xa proteins on the fluorogenic anti-factor Xa assay when monitoring unfractionated heparin

Vanessa Castro-López, Brian Murray, Leanne F. Harris, James S. O'Donnell, Anthony J. Killard

Research output: Contribution to journalArticlepeer-review

Abstract

Four commercially available factor Xa (FXa) reagents were evaluated in a fluorogenic anti-FXa assay. The four reagents - of which three were of human origin and the fourth was bovine - were compared in terms of the resulting assay dynamic ranges, lag times, coefficient of variation and R 2 values, as well as their sensitivity to unfractionated heparin within the therapeutic range of 0-1.2U/ml. Similar performance of reagents in the fluorogenic anti-FXa assay was observed independent of the source of the reagent or its physical state, which may assist in the standardization of coagulation assays in clinical settings.

Original languageEnglish
Pages (from-to)98-103
Number of pages6
JournalBlood Coagulation and Fibrinolysis
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 2012
Externally publishedYes

Keywords

  • anticoagulant monitoring
  • bovine
  • factor Xa
  • fluorogenic antifactor Xa assay
  • human
  • unfractionated heparin

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