Abstract
Four commercially available factor Xa (FXa) reagents were evaluated in a fluorogenic anti-FXa assay. The four reagents - of which three were of human origin and the fourth was bovine - were compared in terms of the resulting assay dynamic ranges, lag times, coefficient of variation and R 2 values, as well as their sensitivity to unfractionated heparin within the therapeutic range of 0-1.2U/ml. Similar performance of reagents in the fluorogenic anti-FXa assay was observed independent of the source of the reagent or its physical state, which may assist in the standardization of coagulation assays in clinical settings.
Original language | English |
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Pages (from-to) | 98-103 |
Number of pages | 6 |
Journal | Blood Coagulation and Fibrinolysis |
Volume | 23 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2012 |
Externally published | Yes |
Keywords
- anticoagulant monitoring
- bovine
- factor Xa
- fluorogenic antifactor Xa assay
- human
- unfractionated heparin