Effect of light on the biodegradation of selective azo dyes by Escherichia coli and Pseudomonas sp. and optimization of conditions for decolorization

Muhammad Kashif, Abdul Jabbar, Abdul Ghaffar, Muhammad Yameen, Bushra Munir

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The degradation of two azo dyes; congo red and acid red 27, commonly used in textile industries, was investigated using Pseudomonassp. and Escherichia coli strains isolated from dye contaminated soil sludge. An aqueous medium containing 100 mg/L of dyes were used for the biodegradation experiments. The degradation results as color removal performance were obtained after 5 days incubation. Different factors like azoreductases specificity, pH and effects of a range of wavelengths of light on decolorization efficiencies were studied. Pseudomonassp. showed highest degradation rate for congo red and Escherichia coli showed highest degradation rate for acid red 27. The color of congo red and acid red 27 dyes were removed up to 98% and 99%, respectively by Pseudomonas sp. and E. coli at the end of anaerobic incubation. Neutral pH was found to be the most appropriate and presence of light showed enhancement of biodegradation rate. Shorting of wavelength of light increased the rate of degradation. Irradiation of UV light before the introduction of microbial strains showed complete color removal only in 2 days period. UV/Visible spectrophotometer was used to monitor the decolorization process.
    Original languageEnglish
    JournalSCIENCE LETTERS
    Volume2
    Issue number1
    Publication statusPublished - 2014

    Keywords

    • Azo dyes
    • congo red
    • acid red 27
    • Escherichia coli
    • Pseudomonas sp
    • Photo-biodegradation

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