TY - JOUR
T1 - Development of support based on chitosan and cellulose nanocrystals for the immobilization of anti-Shiga toxin 2B antibody
AU - Shankar, Shiv
AU - Baraketi, Amina
AU - D'Auria, Sabato
AU - Fraschini, Carole
AU - Salmieri, Stephane
AU - Jamshidian, Majid
AU - Etty, Marie Christine
AU - Lacroix, Monique
N1 - Publisher Copyright:
© 2019
PY - 2020/3/15
Y1 - 2020/3/15
N2 - This work describes the development of membrane based on chitosan (CHI), cellulose nanocrystals (CNCs), and glycerol (GLY), and optimization of the formulation for immobilization of monoclonal anti-Shiga toxin 2B antibody (mAnti-stx2B-Ab) for E. coli O157:H7 detection. The effect of CHI deacetylation degree & viscosity, CNCs and GLY concentrations on Anti-stx2B-Ab immobilization efficiency was evaluated. Fractional factorial and Box-Behnken designs were applied to screen the effects of compounds interactions and optimize their concentrations for detection of Anti-stx2B-Ab. The results demonstrated that the use of 0.6 % (w/v) CNCs improved significantly the Anti-stx2B-Ab immobilization and the level of signal detection. The detection limit of Escherichia coli O157:H7 by developed optimized membrane is 1 log CFU/mL. The time needed for detection of E. coli O157:H7 was only 4 h of enrichment compared to 24 h with conventional methods. The developed immobilization support has potential for future pathogen detection in food and biomedical samples.
AB - This work describes the development of membrane based on chitosan (CHI), cellulose nanocrystals (CNCs), and glycerol (GLY), and optimization of the formulation for immobilization of monoclonal anti-Shiga toxin 2B antibody (mAnti-stx2B-Ab) for E. coli O157:H7 detection. The effect of CHI deacetylation degree & viscosity, CNCs and GLY concentrations on Anti-stx2B-Ab immobilization efficiency was evaluated. Fractional factorial and Box-Behnken designs were applied to screen the effects of compounds interactions and optimize their concentrations for detection of Anti-stx2B-Ab. The results demonstrated that the use of 0.6 % (w/v) CNCs improved significantly the Anti-stx2B-Ab immobilization and the level of signal detection. The detection limit of Escherichia coli O157:H7 by developed optimized membrane is 1 log CFU/mL. The time needed for detection of E. coli O157:H7 was only 4 h of enrichment compared to 24 h with conventional methods. The developed immobilization support has potential for future pathogen detection in food and biomedical samples.
KW - Antibody immobilization
KW - Box-Behnken design
KW - Cellulose nanocrystals
KW - Chitosan
KW - Fractional factorial design
UR - https://www.scopus.com/pages/publications/85077034979
U2 - 10.1016/j.carbpol.2019.115785
DO - 10.1016/j.carbpol.2019.115785
M3 - Article
C2 - 31952593
AN - SCOPUS:85077034979
SN - 0144-8617
VL - 232
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 115785
ER -