Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds

Michael J. Jaasma; Niamh A. Plunkett; Fergal J. O'Brien

doi:10.1016/j.jbiotec.2007.11.010

Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds

Michael J. Jaasma, Niamh A. Plunkett, Fergal J. O'Brien

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

In tissue engineering, flow perfusion bioreactors can be used to enhance nutrient diffusion while mechanically stimulating cells to increase matrix production. The goal of this study was to design and validate a dynamic flow perfusion bioreactor for use with compliant scaffolds. Using a non-permanent staining technique, scaffold perfusion was verified for flow rates of 0.1-2.0 mL/min. Flow analysis revealed that steady, pulsatile and oscillatory flow profiles were effectively transferred from the pump to the scaffold. Compared to static culture, bioreactor culture of osteoblast-seeded collagen-GAG scaffolds led to a 27-34% decrease in cell number but stimulated an 800-1200% increase in the production of prostaglandin E₂, an early-stage bone formation marker. This validated flow perfusion bioreactor provides the basis for optimisation of bioreactor culture in tissue engineering applications.

Original language	English
Pages (from-to)	490-496
Number of pages	7
Journal	Journal of Biotechnology
Volume	133
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiotec.2007.11.010
Publication status	Published - 29 Feb 2008

Keywords

Bioreactor culture
Bone tissue engineering
Cartilage tissue engineering
Fluid flow
Mechanical stimulation

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10.1016/j.jbiotec.2007.11.010

Cite this

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title = "Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds",

abstract = "In tissue engineering, flow perfusion bioreactors can be used to enhance nutrient diffusion while mechanically stimulating cells to increase matrix production. The goal of this study was to design and validate a dynamic flow perfusion bioreactor for use with compliant scaffolds. Using a non-permanent staining technique, scaffold perfusion was verified for flow rates of 0.1-2.0 mL/min. Flow analysis revealed that steady, pulsatile and oscillatory flow profiles were effectively transferred from the pump to the scaffold. Compared to static culture, bioreactor culture of osteoblast-seeded collagen-GAG scaffolds led to a 27-34\% decrease in cell number but stimulated an 800-1200\% increase in the production of prostaglandin E2, an early-stage bone formation marker. This validated flow perfusion bioreactor provides the basis for optimisation of bioreactor culture in tissue engineering applications.",

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AU - Jaasma, Michael J.

AU - Plunkett, Niamh A.

AU - O'Brien, Fergal J.

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AB - In tissue engineering, flow perfusion bioreactors can be used to enhance nutrient diffusion while mechanically stimulating cells to increase matrix production. The goal of this study was to design and validate a dynamic flow perfusion bioreactor for use with compliant scaffolds. Using a non-permanent staining technique, scaffold perfusion was verified for flow rates of 0.1-2.0 mL/min. Flow analysis revealed that steady, pulsatile and oscillatory flow profiles were effectively transferred from the pump to the scaffold. Compared to static culture, bioreactor culture of osteoblast-seeded collagen-GAG scaffolds led to a 27-34% decrease in cell number but stimulated an 800-1200% increase in the production of prostaglandin E2, an early-stage bone formation marker. This validated flow perfusion bioreactor provides the basis for optimisation of bioreactor culture in tissue engineering applications.

KW - Bioreactor culture

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KW - Cartilage tissue engineering

KW - Fluid flow

KW - Mechanical stimulation

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Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds

Abstract

Keywords

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