Counting Platelets: a Novel Image Analysis Algorithm for Detecting and Counting Platelet Adhesion on an Assay Pattern

Changes in platelet function is a major complication in patients with cardiovascular disease. The authors have previously designed a portable, easy to use, low cost biochip for monitoring of anti-platelet therapy in cardiovascular disease patients [1]. This method directly measures platelet function by measuring platelet adhesion to platelet-specific protein patterns using a simple, optical counting technique. The patterned surfaces are designed so that a single platelet adheres to a single protein spot and for imaging purposes, the proteins patterned on the surface are labelled with a fluorescent dye, as are the platelets that adhere to the patterned surface. Following image acquisition, the occupied (i.e. covered by a platelet) protein spots in each sample must be enumerated. The result, calculated as percent adhesion, constitutes a direct and straightforward measurement of platelet adhesion and, therefore, platelet function.