Cloning of mouse genes related to repairing of intestinal epithelium of the γ irradiated mice by treatment with the intestinal RNA of mice of the same strain

To clone the new genes involved in the repair of radiation—damaged intestinal crypts of mice which were treated with the intestinal RNA of the mice of the same strain. As a test group, 45 mice which had been irradiated by γ rays were injected with intestinal RNA of mice of the same strain in 2h after irradiation and the specimens of the small intestine of these animals were collected at 6,12, 24h, 4d and 8d after irradiation respectively. The other 45 mice, as a control group, were treated with irradiation and physiological saline,and the specimens were collected as those in the test group. The genes which expressed more highly in the test group than in the control were cloned into T vectors after subtractive hybridization and LD—PCR and then sequenced. The sequences obtained were aligned through GeneBank for the new gene search. 90 clones were found associate with the repairing of radiation—damaged mouse intestinal crypts, which was confirmed by RNA dot blot assays. Among the 90 clones, 18 were accepted by GeneBank as new genes with the acceptance numbers AF240164—AF240181. Obtained ninety clones may be correlated closely with repairing of intestinal epithelium of the γ —irradiated mice by treatment with the intestinal RNA of mice of the same strain.