Cloning of genes related with exogenous nucleic acids improving the repair of intestinal epithelium after# gamma#-irradiation in mice

IM To explore the molecular mechanism of exogenous nucleic acids improving repair of radiation damaged crypt. METHODS 45 of the mice being irradiated by γ ray were treated with small intestinal RNA as test group, whose small intestinal specimens were collected at 6, 12 and 24 h, 4 d, 8 d after treatment respectively; 45 of the mice being irradiated by γ ray were treated with physiological saline as control group, whose small intestinal specimens were collected. Then genes expressed in test group higher than in control group, were obtained by using LD PCR based on subtractive hybridization. After that, these genes were cloned into T vectors, and then were sequenced. The obtained sequences were searched for GeneBank. RESULTS ①90 of clones associated with repair of radiation damaged crypt were obtained; ②RNA dot blot confirmed that 90 of clones were differentially expressed between test group and control group; ③By searching GeneBank, 18 of 90 genes were new sequences,GeneBank accept number were AF240164 AF240181. CONCLUSION Eighteen genes have been cloned.