TY - JOUR
T1 - Characterization of phenolic composition in lamiaceae spices by LC-ESI-MS/MS
AU - Hossain, Mohammad B.
AU - Rai, Dilip K.
AU - Brunton, Nigel P.
AU - Martin-Diana, Ana B.
AU - Barry-Ryan, And Catherine
PY - 2010/10/13
Y1 - 2010/10/13
N2 - A total of 38 phenolic compounds in the solid/liquid extracts of five Lamiaceae spices, rosemary, oregano, sage, basil, and thyme, were identified in the present study using LC-ESI-MS/MS. These compounds were distributed in four major categories, namely, hydroxycinnamic acid derivatives, hydroxybenzoic acid derivatives, flavonoids, and phenolic terpenes. Among them, the category of flavonoids was the largest, with 17 compounds. Identification of the phenolic compounds was carried out by comparing retention times and mass spectra with those of authentic standards. If standards were unavailable, phenolic compounds were identified on the basis of accurate mass of pseudomolecular [M - H] - ions and tandem mass spectrometry (MS/MS) data. The results of accurate mass measurements fit well with the elemental composition of the compounds. The diagnostic fragmentation patterns of the compounds during collision-induced dissociation (CID) elucidated the structural information of the compounds analyzed.
AB - A total of 38 phenolic compounds in the solid/liquid extracts of five Lamiaceae spices, rosemary, oregano, sage, basil, and thyme, were identified in the present study using LC-ESI-MS/MS. These compounds were distributed in four major categories, namely, hydroxycinnamic acid derivatives, hydroxybenzoic acid derivatives, flavonoids, and phenolic terpenes. Among them, the category of flavonoids was the largest, with 17 compounds. Identification of the phenolic compounds was carried out by comparing retention times and mass spectra with those of authentic standards. If standards were unavailable, phenolic compounds were identified on the basis of accurate mass of pseudomolecular [M - H] - ions and tandem mass spectrometry (MS/MS) data. The results of accurate mass measurements fit well with the elemental composition of the compounds. The diagnostic fragmentation patterns of the compounds during collision-induced dissociation (CID) elucidated the structural information of the compounds analyzed.
KW - Accurate mass
KW - Fragments
KW - LC-ESI-MS/MS
KW - Phenolics
KW - Spice
UR - https://www.scopus.com/pages/publications/78049254083
U2 - 10.1021/jf102042g
DO - 10.1021/jf102042g
M3 - Article
C2 - 20825192
AN - SCOPUS:78049254083
SN - 0021-8561
VL - 58
SP - 10576
EP - 10581
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 19
ER -