Characterization of an interaction between functionalized carbon nanotubes and an enzyme

Marc In Het Panhuis; Carolina Salvador-Morales; Edward Franklin; Gordon Chambers; Antonio Fonseca; Janos B. Nagy; Werner J. Blau; Andrew I. Minett

doi:10.1166/jnn.2003.187

Characterization of an interaction between functionalized carbon nanotubes and an enzyme

Marc In Het Panhuis, Carolina Salvador-Morales, Edward Franklin, Gordon Chambers, Antonio Fonseca, Janos B. Nagy, Werner J. Blau, Andrew I. Minett

Research output: Contribution to journal › Article › peer-review

Abstract

Carbon nanotubes (CNTs) did not exhibit strong interactions with Biliverdin IXB reductase enzyme (BVRB) in water. With the use of noncovalent functionalization by the surfactant Triton X-100, the surfaces of the CNTs were changed from hydrophobic to hydrophilic. The hydrophilic surface of the CNT-Triton conjugate interacts with the hydrophilic surface of BVRB, thus creating a water-soluble complex. Results from ultracentrifugation through a sucrose gradient and gel electrophoresis show the presence of the enzyme. Raman spectroscopy confirmed that the enzyme indeed interacts with CNT-Triton conjugates.

Original language	English
Pages (from-to)	209-213
Number of pages	5
Journal	Journal of Nanoscience and Nanotechnology
Volume	3
Issue number	3
DOIs	https://doi.org/10.1166/jnn.2003.187
Publication status	Published - Jun 2003

Keywords

Carbon nanotubes
Enzymes
Functionalization
Raman
Sensing

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10.1166/jnn.2003.187

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title = "Characterization of an interaction between functionalized carbon nanotubes and an enzyme",

abstract = "Carbon nanotubes (CNTs) did not exhibit strong interactions with Biliverdin IXB reductase enzyme (BVRB) in water. With the use of noncovalent functionalization by the surfactant Triton X-100, the surfaces of the CNTs were changed from hydrophobic to hydrophilic. The hydrophilic surface of the CNT-Triton conjugate interacts with the hydrophilic surface of BVRB, thus creating a water-soluble complex. Results from ultracentrifugation through a sucrose gradient and gel electrophoresis show the presence of the enzyme. Raman spectroscopy confirmed that the enzyme indeed interacts with CNT-Triton conjugates.",

keywords = "Carbon nanotubes, Enzymes, Functionalization, Raman, Sensing",

author = "\{In Het Panhuis\}, Marc and Carolina Salvador-Morales and Edward Franklin and Gordon Chambers and Antonio Fonseca and Nagy, \{Janos B.\} and Blau, \{Werner J.\} and Minett, \{Andrew I.\}",

year = "2003",

month = jun,

doi = "10.1166/jnn.2003.187",

language = "English",

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T1 - Characterization of an interaction between functionalized carbon nanotubes and an enzyme

AU - In Het Panhuis, Marc

AU - Salvador-Morales, Carolina

AU - Franklin, Edward

AU - Chambers, Gordon

AU - Fonseca, Antonio

AU - Nagy, Janos B.

AU - Blau, Werner J.

AU - Minett, Andrew I.

PY - 2003/6

Y1 - 2003/6

N2 - Carbon nanotubes (CNTs) did not exhibit strong interactions with Biliverdin IXB reductase enzyme (BVRB) in water. With the use of noncovalent functionalization by the surfactant Triton X-100, the surfaces of the CNTs were changed from hydrophobic to hydrophilic. The hydrophilic surface of the CNT-Triton conjugate interacts with the hydrophilic surface of BVRB, thus creating a water-soluble complex. Results from ultracentrifugation through a sucrose gradient and gel electrophoresis show the presence of the enzyme. Raman spectroscopy confirmed that the enzyme indeed interacts with CNT-Triton conjugates.

AB - Carbon nanotubes (CNTs) did not exhibit strong interactions with Biliverdin IXB reductase enzyme (BVRB) in water. With the use of noncovalent functionalization by the surfactant Triton X-100, the surfaces of the CNTs were changed from hydrophobic to hydrophilic. The hydrophilic surface of the CNT-Triton conjugate interacts with the hydrophilic surface of BVRB, thus creating a water-soluble complex. Results from ultracentrifugation through a sucrose gradient and gel electrophoresis show the presence of the enzyme. Raman spectroscopy confirmed that the enzyme indeed interacts with CNT-Triton conjugates.

KW - Carbon nanotubes

KW - Enzymes

KW - Functionalization

KW - Raman

KW - Sensing

UR - https://www.scopus.com/pages/publications/0142164532

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DO - 10.1166/jnn.2003.187

M3 - Article

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AN - SCOPUS:0142164532

SN - 1533-4880

VL - 3

SP - 209

EP - 213

JO - Journal of Nanoscience and Nanotechnology

JF - Journal of Nanoscience and Nanotechnology

IS - 3

ER -

Characterization of an interaction between functionalized carbon nanotubes and an enzyme

Abstract

Keywords

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