TY - JOUR
T1 - Cell shape imaging analysis
T2 - A fast and reliable technique for the investigation of internalised carbon nanotubes in flat macrophages
AU - Tian, F.
AU - Prina-Mello, A.
AU - Estrada, G. G.
AU - Beyerle, A.
AU - Kreyling, W.
AU - Stoeger, T.
PY - 2009
Y1 - 2009
N2 - The aim of this work is to elucidate the mechanisms involved in the morphological adaptation and regulation of macrophages in the presence of internalised materials. This development will accelerate the toxicology assessment of novel nanomaterials and subsequently reduce their environmental and health exposure. For this purpose, we adapted our established in vitro culture system to investigate and measure cell shape changes with and without functionalized carbon nanotubes (CNTs). Two nanomaterials, such as fluorescent polystyrene (PS) beads and functionalized CNTs, were employed to track the material location under confocal microscopy, light microscopy and Transmission Electron Microscopy (TEM). It was found that particles equally spread throughout the entire cytoplasm in spherical macrophage; whereas when macrophages where forced to adhere to the substrate, via fibronectin coating, the accumulation of particles and tubes was limited to the vicinity of the nucleus due to the modified cellular micro architecture. TEM analysis also confirmed these findings and demonstrated that CNTs of about 5 |am laid at the bottom of adherent cells. Therefore, this cell shape analysis and manipulation may result very important for the quantification of internalised novel materials with high aspect ratio like nanotubes, nanorods and nanowires.
AB - The aim of this work is to elucidate the mechanisms involved in the morphological adaptation and regulation of macrophages in the presence of internalised materials. This development will accelerate the toxicology assessment of novel nanomaterials and subsequently reduce their environmental and health exposure. For this purpose, we adapted our established in vitro culture system to investigate and measure cell shape changes with and without functionalized carbon nanotubes (CNTs). Two nanomaterials, such as fluorescent polystyrene (PS) beads and functionalized CNTs, were employed to track the material location under confocal microscopy, light microscopy and Transmission Electron Microscopy (TEM). It was found that particles equally spread throughout the entire cytoplasm in spherical macrophage; whereas when macrophages where forced to adhere to the substrate, via fibronectin coating, the accumulation of particles and tubes was limited to the vicinity of the nucleus due to the modified cellular micro architecture. TEM analysis also confirmed these findings and demonstrated that CNTs of about 5 |am laid at the bottom of adherent cells. Therefore, this cell shape analysis and manipulation may result very important for the quantification of internalised novel materials with high aspect ratio like nanotubes, nanorods and nanowires.
UR - http://www.scopus.com/inward/record.url?scp=65649132355&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/151/1/012033
DO - 10.1088/1742-6596/151/1/012033
M3 - Article
AN - SCOPUS:65649132355
SN - 1742-6588
VL - 151
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
M1 - 012033
ER -