Carbon nanotube-based transducers for immunoassays

Carol Lynam; Niamh Gilmartin; Andrew I. Minett; Richard O'Kennedy; Gordon Wallace

doi:10.1016/j.carbon.2009.04.017

Carbon nanotube-based transducers for immunoassays

Carol Lynam, Niamh Gilmartin, Andrew I. Minett, Richard O'Kennedy, Gordon Wallace

Research output: Contribution to journal › Article › peer-review

Abstract

The attachment of mouse immunoglobulin G (IgG) and anti-mouse IgG antibodies onto carbon nanotubes (CNTs), using either non-covalent or covalent means was investigated. The resultant CNTs were characterised using a variety of techniques including enzyme-linked and fluorescence-linked immunoassays, UV-visible-NIR and Raman spectroscopy, transmission electron microscopy and cyclic voltammetry. TEM images of the adsorbed antibody on the CNTs show that the covalent modification approach was successful, whereas the non-covalent approach resulted in no electrochemically detectable labelled antibody. Direct electrical communication between CNTs covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which suggests applications in developing carbon-nanotube-based immunosensors.

Original language	English
Pages (from-to)	2337-2343
Number of pages	7
Journal	Carbon
Volume	47
Issue number	10
DOIs	https://doi.org/10.1016/j.carbon.2009.04.017
Publication status	Published - Aug 2009

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title = "Carbon nanotube-based transducers for immunoassays",

abstract = "The attachment of mouse immunoglobulin G (IgG) and anti-mouse IgG antibodies onto carbon nanotubes (CNTs), using either non-covalent or covalent means was investigated. The resultant CNTs were characterised using a variety of techniques including enzyme-linked and fluorescence-linked immunoassays, UV-visible-NIR and Raman spectroscopy, transmission electron microscopy and cyclic voltammetry. TEM images of the adsorbed antibody on the CNTs show that the covalent modification approach was successful, whereas the non-covalent approach resulted in no electrochemically detectable labelled antibody. Direct electrical communication between CNTs covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which suggests applications in developing carbon-nanotube-based immunosensors.",

author = "Carol Lynam and Niamh Gilmartin and Minett, \{Andrew I.\} and Richard O'Kennedy and Gordon Wallace",

year = "2009",

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doi = "10.1016/j.carbon.2009.04.017",

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AU - Gilmartin, Niamh

AU - Minett, Andrew I.

AU - O'Kennedy, Richard

AU - Wallace, Gordon

PY - 2009/8

Y1 - 2009/8

N2 - The attachment of mouse immunoglobulin G (IgG) and anti-mouse IgG antibodies onto carbon nanotubes (CNTs), using either non-covalent or covalent means was investigated. The resultant CNTs were characterised using a variety of techniques including enzyme-linked and fluorescence-linked immunoassays, UV-visible-NIR and Raman spectroscopy, transmission electron microscopy and cyclic voltammetry. TEM images of the adsorbed antibody on the CNTs show that the covalent modification approach was successful, whereas the non-covalent approach resulted in no electrochemically detectable labelled antibody. Direct electrical communication between CNTs covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which suggests applications in developing carbon-nanotube-based immunosensors.

AB - The attachment of mouse immunoglobulin G (IgG) and anti-mouse IgG antibodies onto carbon nanotubes (CNTs), using either non-covalent or covalent means was investigated. The resultant CNTs were characterised using a variety of techniques including enzyme-linked and fluorescence-linked immunoassays, UV-visible-NIR and Raman spectroscopy, transmission electron microscopy and cyclic voltammetry. TEM images of the adsorbed antibody on the CNTs show that the covalent modification approach was successful, whereas the non-covalent approach resulted in no electrochemically detectable labelled antibody. Direct electrical communication between CNTs covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which suggests applications in developing carbon-nanotube-based immunosensors.

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SN - 0008-6223

VL - 47

SP - 2337

EP - 2343

JO - Carbon

JF - Carbon

IS - 10

ER -

Carbon nanotube-based transducers for immunoassays

Abstract

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