ATR-Spin: An open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals

David Pérez-Guaita; Zack Richardson; Guillermo Quintás; Julia Kuligowski; Diana E. Bedolla; Hugh J. Byrne; Bayden Wood

doi:10.1039/d1lc00813g

ATR-Spin: An open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals

David Pérez-Guaita, Zack Richardson, Guillermo Quintás, Julia Kuligowski, Diana E. Bedolla, Hugh J. Byrne, Bayden Wood

Physical to Life Sciences Research Hub

Research output: Contribution to journal › Article › peer-review

Abstract

Infrared spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of point-of-care diagnostics for cell analysis. Here we introduce an open-source, 3D printed device for integrating the isolation, preconcentration, and measurement of attenuated total reflectance IR spectra of cells from biofluids. The tool comprises a disposable card for cytocentrifugation, equipped with magnets, which allows reproducible integration into the pathlength of the IR spectrophotometer. Preliminary results using cell culture media containing A549 cells indicate that this system enables a qualitative and quantitative characterization of cells down to 10 cells μL-1 by using a single and cost-effective device and within a few minutes.

Original language	English
Pages (from-to)	4743-4748
Number of pages	6
Journal	Lab on a Chip
Volume	21
Issue number	24
DOIs	https://doi.org/10.1039/d1lc00813g
Publication status	Published - 21 Dec 2021

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title = "ATR-Spin: An open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals",

abstract = "Infrared spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of point-of-care diagnostics for cell analysis. Here we introduce an open-source, 3D printed device for integrating the isolation, preconcentration, and measurement of attenuated total reflectance IR spectra of cells from biofluids. The tool comprises a disposable card for cytocentrifugation, equipped with magnets, which allows reproducible integration into the pathlength of the IR spectrophotometer. Preliminary results using cell culture media containing A549 cells indicate that this system enables a qualitative and quantitative characterization of cells down to 10 cells μL-1 by using a single and cost-effective device and within a few minutes.",

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AU - Pérez-Guaita, David

AU - Richardson, Zack

AU - Quintás, Guillermo

AU - Kuligowski, Julia

AU - Bedolla, Diana E.

AU - Byrne, Hugh J.

AU - Wood, Bayden

PY - 2021/12/21

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AB - Infrared spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of point-of-care diagnostics for cell analysis. Here we introduce an open-source, 3D printed device for integrating the isolation, preconcentration, and measurement of attenuated total reflectance IR spectra of cells from biofluids. The tool comprises a disposable card for cytocentrifugation, equipped with magnets, which allows reproducible integration into the pathlength of the IR spectrophotometer. Preliminary results using cell culture media containing A549 cells indicate that this system enables a qualitative and quantitative characterization of cells down to 10 cells μL-1 by using a single and cost-effective device and within a few minutes.

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ATR-Spin: An open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals

Abstract

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