Antiadhesive glycoconjugate metal complexes targeting pathogens Pseudomonas aeruginosa and Candida albicans

Karolina Wojtczak; Emilie Gillon; Diana Bura; Karen Richmond; Megan Joyce; Emma Caraher; Keela Kessie; Trinidad Velasco-Torrijos; Cristina Trujillo; Anne Imberty; Kevin Kavanagh; Gordon Cooke; Joseph P. Byrne

doi:10.1039/d5ob00970g

Antiadhesive glycoconjugate metal complexes targeting pathogens Pseudomonas aeruginosa and Candida albicans

Karolina Wojtczak, Emilie Gillon, Diana Bura, Karen Richmond, Megan Joyce, Emma Caraher, Keela Kessie, Trinidad Velasco-Torrijos, Cristina Trujillo, Anne Imberty, Kevin Kavanagh, Gordon Cooke, Joseph P. Byrne

Research output: Contribution to journal › Article › peer-review

Abstract

Glycoconjugates are known to interact with carbohydrate-binding proteins involved in adhesion by pathogens, and offer opportunities to design antimicrobial agents. Metal complexes with Eu(iii), Ni(ii) and Zn(ii) were prepared from glycoconjugate ligand 1Gal, which binds to P. aeruginosa's lectin LecA (K_d 9.6 ± 0.7 μM). In vitro anti-adhesive activity of these compounds was evaluated for both P. aeruginosa and C. albicans. Choice of metal ion played a crucial role in modulating anti-adhesive activity, with Eu(iii) complexes most effective: [Eu·(1Gal)(H₂O)₆](CF₃SO₃)₃ inhibits 60% biofilm formation by P. aeruginosa and [Eu·(1Gal)₃](CF₃SO₃)₃ inhibits 62% of C. albicans adhesion to buccal epithelial cells (both at 0.1 mM). The results presented demonstrate the potential for metal coordination to significantly enhance biological activity of glycoconjugates, surpassing the effect of the ligand's modest lectin-binding affinity alone.

Original language	English
Journal	Organic and Biomolecular Chemistry
DOIs	https://doi.org/10.1039/d5ob00970g
Publication status	Accepted/In press - 2025

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Wojtczak, K., Gillon, E., Bura, D., Richmond, K., Joyce, M., Caraher, E., Kessie, K., Velasco-Torrijos, T., Trujillo, C., Imberty, A., Kavanagh, K., Cooke, G., & Byrne, J. P. (Accepted/In press). Antiadhesive glycoconjugate metal complexes targeting pathogens Pseudomonas aeruginosa and Candida albicans. Organic and Biomolecular Chemistry. https://doi.org/10.1039/d5ob00970g

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title = "Antiadhesive glycoconjugate metal complexes targeting pathogens Pseudomonas aeruginosa and Candida albicans",

abstract = "Glycoconjugates are known to interact with carbohydrate-binding proteins involved in adhesion by pathogens, and offer opportunities to design antimicrobial agents. Metal complexes with Eu(iii), Ni(ii) and Zn(ii) were prepared from glycoconjugate ligand 1Gal, which binds to P. aeruginosa's lectin LecA (Kd 9.6 ± 0.7 μM). In vitro anti-adhesive activity of these compounds was evaluated for both P. aeruginosa and C. albicans. Choice of metal ion played a crucial role in modulating anti-adhesive activity, with Eu(iii) complexes most effective: [Eu·(1Gal)(H2O)6](CF3SO3)3 inhibits 60\% biofilm formation by P. aeruginosa and [Eu·(1Gal)3](CF3SO3)3 inhibits 62\% of C. albicans adhesion to buccal epithelial cells (both at 0.1 mM). The results presented demonstrate the potential for metal coordination to significantly enhance biological activity of glycoconjugates, surpassing the effect of the ligand's modest lectin-binding affinity alone.",

author = "Karolina Wojtczak and Emilie Gillon and Diana Bura and Karen Richmond and Megan Joyce and Emma Caraher and Keela Kessie and Trinidad Velasco-Torrijos and Cristina Trujillo and Anne Imberty and Kevin Kavanagh and Gordon Cooke and Byrne, \{Joseph P.\}",

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year = "2025",

doi = "10.1039/d5ob00970g",

language = "English",

journal = "Organic and Biomolecular Chemistry",

issn = "1477-0520",

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T1 - Antiadhesive glycoconjugate metal complexes targeting pathogens Pseudomonas aeruginosa and Candida albicans

AU - Wojtczak, Karolina

AU - Gillon, Emilie

AU - Bura, Diana

AU - Richmond, Karen

AU - Joyce, Megan

AU - Caraher, Emma

AU - Kessie, Keela

AU - Velasco-Torrijos, Trinidad

AU - Trujillo, Cristina

AU - Imberty, Anne

AU - Kavanagh, Kevin

AU - Cooke, Gordon

AU - Byrne, Joseph P.

PY - 2025

Y1 - 2025

N2 - Glycoconjugates are known to interact with carbohydrate-binding proteins involved in adhesion by pathogens, and offer opportunities to design antimicrobial agents. Metal complexes with Eu(iii), Ni(ii) and Zn(ii) were prepared from glycoconjugate ligand 1Gal, which binds to P. aeruginosa's lectin LecA (Kd 9.6 ± 0.7 μM). In vitro anti-adhesive activity of these compounds was evaluated for both P. aeruginosa and C. albicans. Choice of metal ion played a crucial role in modulating anti-adhesive activity, with Eu(iii) complexes most effective: [Eu·(1Gal)(H2O)6](CF3SO3)3 inhibits 60% biofilm formation by P. aeruginosa and [Eu·(1Gal)3](CF3SO3)3 inhibits 62% of C. albicans adhesion to buccal epithelial cells (both at 0.1 mM). The results presented demonstrate the potential for metal coordination to significantly enhance biological activity of glycoconjugates, surpassing the effect of the ligand's modest lectin-binding affinity alone.

AB - Glycoconjugates are known to interact with carbohydrate-binding proteins involved in adhesion by pathogens, and offer opportunities to design antimicrobial agents. Metal complexes with Eu(iii), Ni(ii) and Zn(ii) were prepared from glycoconjugate ligand 1Gal, which binds to P. aeruginosa's lectin LecA (Kd 9.6 ± 0.7 μM). In vitro anti-adhesive activity of these compounds was evaluated for both P. aeruginosa and C. albicans. Choice of metal ion played a crucial role in modulating anti-adhesive activity, with Eu(iii) complexes most effective: [Eu·(1Gal)(H2O)6](CF3SO3)3 inhibits 60% biofilm formation by P. aeruginosa and [Eu·(1Gal)3](CF3SO3)3 inhibits 62% of C. albicans adhesion to buccal epithelial cells (both at 0.1 mM). The results presented demonstrate the potential for metal coordination to significantly enhance biological activity of glycoconjugates, surpassing the effect of the ligand's modest lectin-binding affinity alone.

UR - https://www.scopus.com/pages/publications/105009590294

U2 - 10.1039/d5ob00970g

DO - 10.1039/d5ob00970g

M3 - Article

AN - SCOPUS:105009590294

SN - 1477-0520

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

ER -

Antiadhesive glycoconjugate metal complexes targeting pathogens Pseudomonas aeruginosa and Candida albicans

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