TY - JOUR
T1 - Advancing Raman microspectroscopy for cellular and subcellular analysis
T2 - towards in vitro high-content spectralomic analysis
AU - Byrne, Hugh J.
AU - Bonnier, Franck
AU - Casey, Alan
AU - Maher, Marcus
AU - McIntyre, Jennifer
AU - Efeoglu, Esen
AU - Farhane, Zeineb
N1 - Publisher Copyright:
© 2018 Optical Society of America.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - In the confocal mode, Raman microspectroscopy can profile the biochemical content of biological cells at a subcellular level, and any changes to it by exogenous agents, such as therapeutic drugs or toxicants. As an exploration of the potential of the technique as a high-content, label-free analysis technique, this report reviews work to monitor the spectroscopic signatures associated with the uptake and response pathways of commercial chemotherapeutic agents and polymeric nanoparticles by human lung cells. It is demonstrated that the signatures are reproducible and characteristic of the cellular event, and can be used, for example, to identify the mode of action of the agent as well as the subsequent cell death pathway, and even mechanisms of cellular resistance. Data mining approaches are discussed and a spectralomics approach is proposed.
AB - In the confocal mode, Raman microspectroscopy can profile the biochemical content of biological cells at a subcellular level, and any changes to it by exogenous agents, such as therapeutic drugs or toxicants. As an exploration of the potential of the technique as a high-content, label-free analysis technique, this report reviews work to monitor the spectroscopic signatures associated with the uptake and response pathways of commercial chemotherapeutic agents and polymeric nanoparticles by human lung cells. It is demonstrated that the signatures are reproducible and characteristic of the cellular event, and can be used, for example, to identify the mode of action of the agent as well as the subsequent cell death pathway, and even mechanisms of cellular resistance. Data mining approaches are discussed and a spectralomics approach is proposed.
UR - http://www.scopus.com/inward/record.url?scp=85051263019&partnerID=8YFLogxK
U2 - 10.1364/AO.57.000E11
DO - 10.1364/AO.57.000E11
M3 - Article
C2 - 30117916
AN - SCOPUS:85051263019
SN - 1559-128X
VL - 57
SP - E11-E19
JO - Applied Optics
JF - Applied Optics
IS - 22
ER -