TY - JOUR
T1 - A pilot study for early detection of oral premalignant diseases using oral cytology and Raman micro-spectroscopy
T2 - Assessment of confounding factors
AU - Behl, Isha
AU - Calado, Genecy
AU - Malkin, Alison
AU - Flint, Stephen
AU - Galvin, Sheila
AU - Healy, Claire M.
AU - Pimentel, Marina Leite
AU - Byrne, Hugh J.
AU - Lyng, Fiona M.
N1 - Publisher Copyright:
© 2020 WILEY-VCH GmbH
PY - 2020/11/1
Y1 - 2020/11/1
N2 - This study demonstrates the efficacy of Raman micro-spectroscopy of oral cytological samples for differentiating dysplastic, potentially malignant lesions from those of normal, healthy donors. Cells were collected using brush biopsy from healthy donors (n = 20) and patients attending a Dysplasia Clinic (n = 20). Donors were sampled at four different sites (buccal mucosa, tongue, alveolus, gingiva), to ensure matched normal sites for all lesions, while patient samples were taken from clinically evident, histologically verified dysplastic lesions. Spectra were acquired from the nucleus and cytoplasm of individual cells of all samples and subjected to partial least squares-discriminant analysis. Discriminative sensitivities of 94% and 86% and specificity of 85% were achieved for the cytoplasm and nucleus, respectively, largely based on lipidic contributions of dysplastic cells. Alveolar/gingival samples were differentiated from tongue/buccal samples, indicating that anatomical site is potentially a confounding factor, while age, gender, smoking and alcohol consumption were confirmed not to be.
AB - This study demonstrates the efficacy of Raman micro-spectroscopy of oral cytological samples for differentiating dysplastic, potentially malignant lesions from those of normal, healthy donors. Cells were collected using brush biopsy from healthy donors (n = 20) and patients attending a Dysplasia Clinic (n = 20). Donors were sampled at four different sites (buccal mucosa, tongue, alveolus, gingiva), to ensure matched normal sites for all lesions, while patient samples were taken from clinically evident, histologically verified dysplastic lesions. Spectra were acquired from the nucleus and cytoplasm of individual cells of all samples and subjected to partial least squares-discriminant analysis. Discriminative sensitivities of 94% and 86% and specificity of 85% were achieved for the cytoplasm and nucleus, respectively, largely based on lipidic contributions of dysplastic cells. Alveolar/gingival samples were differentiated from tongue/buccal samples, indicating that anatomical site is potentially a confounding factor, while age, gender, smoking and alcohol consumption were confirmed not to be.
KW - Raman micro-spectroscopy
KW - oral brush biopsy cytological samples
KW - oral potentially malignant lesions
KW - partial least squares-discriminant analysis
KW - sensitivity and specificity
UR - http://www.scopus.com/inward/record.url?scp=85088827854&partnerID=8YFLogxK
U2 - 10.1002/jbio.202000079
DO - 10.1002/jbio.202000079
M3 - Article
C2 - 32686263
AN - SCOPUS:85088827854
SN - 1864-063X
VL - 13
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 11
M1 - e202000079
ER -