A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling

Xolile Fuku; Baljit Singh; Rachel F. Ajayi; Abongile N. Jijana; Priscilla Baker; Eithne Dempsey; Emmanuel Iwuoha

doi:10.1039/c5ay00962f

A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling

Xolile Fuku, Baljit Singh, Rachel F. Ajayi, Abongile N. Jijana, Priscilla Baker, Eithne Dempsey, Emmanuel Iwuoha

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Research output: Contribution to journal › Article › peer-review

Abstract

Human epidermal growth factor receptor 2 (Her2/neu) is a biomarker that is overexpressed in human breast cancers. A quantum dots (QDs) -based genosensor for Her2/neu oncogene was developed with gallium telluride QDs and amine-terminated probe ssDNA (NH₂-5′-AATTCCAGTGGCCATCAA-3′), that is complementary to the DNA sequence of a section of the ERF gene of HER-2/neu (i.e. 5′-GAACATGAAGGACCGGTGGGC-3′). The QDs were highly crystalline, 6 nm (from XRD) in size and had band gap values of 3.3 eV and 3.5 eV calculated from fluorescence emission and UV-visible absorption data, respectively. The sensor sensitivity and limit of detection values were 10.0 μA ng^-1 mL^-1 and 0.2 pg mL^-1 Her2/neu oncogene, respectively.

Original language	English
Pages (from-to)	6114-6124
Number of pages	11
Journal	Analytical Methods
Volume	7
Issue number	15
DOIs	https://doi.org/10.1039/c5ay00962f https://doi.org/10.1039/c5ay00962f
Publication status	Published - 7 Aug 2015

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@article{1052c2f496734a88a33fa14aad4978c5,

title = "A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling",

abstract = "Human epidermal growth factor receptor 2 (Her2/neu) is a biomarker that is overexpressed in human breast cancers. A quantum dots (QDs) -based genosensor for Her2/neu oncogene was developed with gallium telluride QDs and amine-terminated probe ssDNA (NH2-5′-AATTCCAGTGGCCATCAA-3′), that is complementary to the DNA sequence of a section of the ERF gene of HER-2/neu (i.e. 5′-GAACATGAAGGACCGGTGGGC-3′). The QDs were highly crystalline, 6 nm (from XRD) in size and had band gap values of 3.3 eV and 3.5 eV calculated from fluorescence emission and UV-visible absorption data, respectively. The sensor sensitivity and limit of detection values were 10.0 μA ng-1 mL-1 and 0.2 pg mL-1 Her2/neu oncogene, respectively.",

author = "Xolile Fuku and Baljit Singh and Ajayi, \{Rachel F.\} and Jijana, \{Abongile N.\} and Priscilla Baker and Eithne Dempsey and Emmanuel Iwuoha",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2015.",

year = "2015",

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doi = "10.1039/c5ay00962f",

language = "English",

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T1 - A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling

AU - Fuku, Xolile

AU - Singh, Baljit

AU - Ajayi, Rachel F.

AU - Jijana, Abongile N.

AU - Baker, Priscilla

AU - Dempsey, Eithne

AU - Iwuoha, Emmanuel

PY - 2015/8/7

Y1 - 2015/8/7

N2 - Human epidermal growth factor receptor 2 (Her2/neu) is a biomarker that is overexpressed in human breast cancers. A quantum dots (QDs) -based genosensor for Her2/neu oncogene was developed with gallium telluride QDs and amine-terminated probe ssDNA (NH2-5′-AATTCCAGTGGCCATCAA-3′), that is complementary to the DNA sequence of a section of the ERF gene of HER-2/neu (i.e. 5′-GAACATGAAGGACCGGTGGGC-3′). The QDs were highly crystalline, 6 nm (from XRD) in size and had band gap values of 3.3 eV and 3.5 eV calculated from fluorescence emission and UV-visible absorption data, respectively. The sensor sensitivity and limit of detection values were 10.0 μA ng-1 mL-1 and 0.2 pg mL-1 Her2/neu oncogene, respectively.

AB - Human epidermal growth factor receptor 2 (Her2/neu) is a biomarker that is overexpressed in human breast cancers. A quantum dots (QDs) -based genosensor for Her2/neu oncogene was developed with gallium telluride QDs and amine-terminated probe ssDNA (NH2-5′-AATTCCAGTGGCCATCAA-3′), that is complementary to the DNA sequence of a section of the ERF gene of HER-2/neu (i.e. 5′-GAACATGAAGGACCGGTGGGC-3′). The QDs were highly crystalline, 6 nm (from XRD) in size and had band gap values of 3.3 eV and 3.5 eV calculated from fluorescence emission and UV-visible absorption data, respectively. The sensor sensitivity and limit of detection values were 10.0 μA ng-1 mL-1 and 0.2 pg mL-1 Her2/neu oncogene, respectively.

UR - https://www.scopus.com/pages/publications/84937920223

U2 - 10.1039/c5ay00962f

DO - 10.1039/c5ay00962f

M3 - Article

AN - SCOPUS:84937920223

SN - 1759-9660

VL - 7

SP - 6114

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JO - Analytical Methods

JF - Analytical Methods

IS - 15

ER -

A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling

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