A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling

Xolile Fuku; Baljit Singh; Rachel F. Ajayi; Abongile N. Jijana; Priscilla Baker; Eithne Dempsey; Emmanuel Iwuoha

doi:10.1039/c5ay00962f

A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling

Xolile Fuku, Baljit Singh, Rachel F. Ajayi, Abongile N. Jijana, Priscilla Baker, Eithne Dempsey, Emmanuel Iwuoha

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Abstract

Human epidermal growth factor receptor 2 (Her2/neu) is a biomarker that is overexpressed in human breast cancers. A quantum dots (QDs) -based genosensor for Her2/neu oncogene was developed with gallium telluride QDs and amine-terminated probe ssDNA (NH₂-5′-AATTCCAGTGGCCATCAA-3′), that is complementary to the DNA sequence of a section of the ERF gene of HER-2/neu (i.e. 5′-GAACATGAAGGACCGGTGGGC-3′). The QDs were highly crystalline, 6 nm (from XRD) in size and had band gap values of 3.3 eV and 3.5 eV calculated from fluorescence emission and UV-visible absorption data, respectively. The sensor sensitivity and limit of detection values were 10.0 μA ng^-1 mL^-1 and 0.2 pg mL^-1 Her2/neu oncogene, respectively.

Original language	English
Pages (from-to)	6114-6124
Number of pages	11
Journal	Analytical Methods
Volume	7
Issue number	15
DOIs	https://doi.org/10.1039/c5ay00962f https://doi.org/10.1039/c5ay00962f
Publication status	Published - 7 Aug 2015

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title = "A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling",

abstract = "Human epidermal growth factor receptor 2 (Her2/neu) is a biomarker that is overexpressed in human breast cancers. A quantum dots (QDs) -based genosensor for Her2/neu oncogene was developed with gallium telluride QDs and amine-terminated probe ssDNA (NH2-5′-AATTCCAGTGGCCATCAA-3′), that is complementary to the DNA sequence of a section of the ERF gene of HER-2/neu (i.e. 5′-GAACATGAAGGACCGGTGGGC-3′). The QDs were highly crystalline, 6 nm (from XRD) in size and had band gap values of 3.3 eV and 3.5 eV calculated from fluorescence emission and UV-visible absorption data, respectively. The sensor sensitivity and limit of detection values were 10.0 μA ng-1 mL-1 and 0.2 pg mL-1 Her2/neu oncogene, respectively.",

author = "Xolile Fuku and Baljit Singh and Ajayi, {Rachel F.} and Jijana, {Abongile N.} and Priscilla Baker and Eithne Dempsey and Emmanuel Iwuoha",

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AU - Singh, Baljit

AU - Ajayi, Rachel F.

AU - Jijana, Abongile N.

AU - Baker, Priscilla

AU - Dempsey, Eithne

AU - Iwuoha, Emmanuel

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AB - Human epidermal growth factor receptor 2 (Her2/neu) is a biomarker that is overexpressed in human breast cancers. A quantum dots (QDs) -based genosensor for Her2/neu oncogene was developed with gallium telluride QDs and amine-terminated probe ssDNA (NH2-5′-AATTCCAGTGGCCATCAA-3′), that is complementary to the DNA sequence of a section of the ERF gene of HER-2/neu (i.e. 5′-GAACATGAAGGACCGGTGGGC-3′). The QDs were highly crystalline, 6 nm (from XRD) in size and had band gap values of 3.3 eV and 3.5 eV calculated from fluorescence emission and UV-visible absorption data, respectively. The sensor sensitivity and limit of detection values were 10.0 μA ng-1 mL-1 and 0.2 pg mL-1 Her2/neu oncogene, respectively.

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JO - Analytical Methods

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ER -

A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling

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