TY - JOUR
T1 - A comparative analysis of pretreatment strategies on the properties and hydrolysis of brewers’ spent grain
AU - Ravindran, Rajeev
AU - Jaiswal, Swarna
AU - Abu-Ghannam, Nissreen
AU - Jaiswal, Amit K.
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2018/1
Y1 - 2018/1
N2 - In this study, brewer's spent grain (BSG) was subjected to a range pretreatments to study the effect on reducing sugar yield. Glucose and xylose were found to be the predominant sugars in BSG. Brewers spent grain was high in cellulose (19.21 g/100 g of BSG) and lignin content (30.84 g/100 g of BSG). Microwave assisted alkali (MAA) pretreatment was found to be the most effective pretreatment for BSG, where the pretreatment was conducted at 400 W for 60 s. A maximum reducing yield was observed with high biomass loading (1 g/10 ml), cellulase (158.76 μl/10 ml), hemicellulase (153.3 μl/10 ml), pH (5.4) and an incubation time (120 h). Upon enzymatic hydrolysis, MAA pretreated BSG yielded 228.25 mg of reducing sugar/g of BSG which was 2.86-fold higher compared to native BSG (79.67 mg/g of BSG); simultaneously BSG was de-lignified significantly. The changes in functional groups, crystallinity and thermal behaviour was studies by means of FTIR, XRD and DSC, respectively.
AB - In this study, brewer's spent grain (BSG) was subjected to a range pretreatments to study the effect on reducing sugar yield. Glucose and xylose were found to be the predominant sugars in BSG. Brewers spent grain was high in cellulose (19.21 g/100 g of BSG) and lignin content (30.84 g/100 g of BSG). Microwave assisted alkali (MAA) pretreatment was found to be the most effective pretreatment for BSG, where the pretreatment was conducted at 400 W for 60 s. A maximum reducing yield was observed with high biomass loading (1 g/10 ml), cellulase (158.76 μl/10 ml), hemicellulase (153.3 μl/10 ml), pH (5.4) and an incubation time (120 h). Upon enzymatic hydrolysis, MAA pretreated BSG yielded 228.25 mg of reducing sugar/g of BSG which was 2.86-fold higher compared to native BSG (79.67 mg/g of BSG); simultaneously BSG was de-lignified significantly. The changes in functional groups, crystallinity and thermal behaviour was studies by means of FTIR, XRD and DSC, respectively.
KW - Biomass pretreatment
KW - Brewer's spent grain
KW - Lignocellulose
KW - Microwave assisted alkali pretreatment
KW - Reducing sugar
UR - http://www.scopus.com/inward/record.url?scp=85021078755&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2017.06.039
DO - 10.1016/j.biortech.2017.06.039
M3 - Article
C2 - 28648256
AN - SCOPUS:85021078755
SN - 0960-8524
VL - 248
SP - 272
EP - 279
JO - Bioresource Technology
JF - Bioresource Technology
ER -